ANTIOXIDANT AND HEPATOPROTECTIVE EFFECT OF SWERTIA CHIRATA ON HYPOXIA-INDUCED OXIDATIVE STRESS IN WISTAR RATS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 1
Abstract
Objective: Swertia chirata has been used in traditional and folklore medicine to treat several ailments such as hepatic disorders. However, the mechanistic and experimental justification to its traditional use is lacking. The present study was aimed to investigate the hepatoprotective potential of S. chirata during hypoxia (HYP)-induced hepatic damage in Wistar rats and to determine the underlying mechanism.Methods: Hydroalcoholic extract of S. chirata was prepared using Soxhlet extraction. Animals were divided into six groups (n=5). Animals in the HYP groups were subjected to HYP for 3 days (10% O2) to induce oxidative stress and hepatic damage. 50 and 100 mg/kg extract treatments were provided orally once daily for 7 days after which animals were sacrificed, and biochemical investigations for oxidative stress, liver function tests, and hepatic histopathology were performed.Results: HYP-induced marked oxidative stress as indicated by the significantly elevated mitochondrial ROS generation, lipid peroxidation, glutathione, and depleted catalase levels. Liver function test indicated hepatic damage as the levels of serum glutamic-oxaloacetic transaminase, serum glutamic pyruvic transaminase, and aspartate transaminase were significantly elevated in HYP animals. S. chirata treatment alleviated oxidative stress and improved liver functions in a dose-dependent manner. Liver histopathology confirmed the marked hepatic damage induced by HYP and revealed that S. chirata efficiently rescued liver from hypoxic damage.Conclusion: Hydroalcoholic extract of S. chirata is a potent hepatoprotective intervention which was associated with its potential to alleviate oxidative stress and improve liver functions. Moreover, it could find clinical application as a safer and alternative remedy for liver ailments.
Authors and Affiliations
Kritika Kaushal, Harvinder Singh, Anil Kant Thakur
Keywords
Related Articles
- EP ID EP607098
- DOI 10.22159/ajpcr.2019.v12i1.28502
- Views 138
- Downloads 0
HAVING A GO AT SPINAL MUSCULAR ATROPHY WITH SPINRAZA
Spinal muscular atrophy (SMA), a neurological condition which is genetically mediated is the second most common infantile disease causing morbidity and mortality next to cystic fibrosis. It is of five different types wit...
In vitro cytotoxic and apoptotic activity of polysaccharide rich Morinda citrofolia fruit on MCF-7 cells
Morinda citrofolia fruit or noni is well-known traditional medicine that has both antioxidant and anticancer activity. This study was designed toinvestigate experimentally the anti-apoptotic effect of polysaccharide isol...
BLOOD PRESSURE LOWERING EFFECT OF POLYHERBAL PREPARATION CONTAINING ALLIUM SATIVUM, BELERICAE FRUCTUS, CURCUMA AERUGINOSA, AND AMOMI FRUCTUS ON RAT MODEL OF HYPERTENSION
Objective: The study aimed to investigate the blood pressure lowering effect of the polyherbal preparation contains Allium sativum, Belericae fructus, Curcuma aeruginosa, and Amomi fructus in the animal model of hyperten...
EFFECTS OF ETHANOL CINCAU (CYCLEA BARBATA MIERS) LEAVES EXTRACT ON MACROPHAGE MIGRATION ON BURN WOUND
Objective: Macrophages are essential cells in burn wound healing. The primary function of macrophages is phagocytosis of microbes and necrosis tissue. Migration of macrophages into the wound area is affected by interleuk...
DEVELOPMENT AND VALIDATION OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF NEBIVOLOL HYDROCHLORIDE AND CILNIDIPINE
Objective: The proposed method describes method development and validation of nebivolol hydrochloride and cilnidipine in combined pharmaceutical tablet dosage form by high-performance thin-layer chromatography (HPTLC) ha...