AUTOLYSIS OF BAKER’S YEAST AND PARTIAL PURIFICATION OF INVERTASE IN THE PRESENCE OF SURFACTANTS
Journal Title: European Journal of Pharmaceutical and Medical Research - Year 2019, Vol 6, Issue 5
Abstract
Baker’s yeast cells were suspended in 0.154M NaHCO3 in the presence or absence of surfactants (TWEEN 20, 40, 60, or 80), and shaken at 200 cycles/min at 45oC for 24 h. The suspension was centrifuged (10,000 rpm/20 min) and the supernatant collected (autolyzate). Sodium picrate was added to the autolyzate; after 5h of contact at room temperature, the precipitate formed was separated by centrifugation (10,000 rpm/30 min) and discharged. The supernatant was treated with acetone in the presence or absence of surfactants to precipitate invertase. Invertase activity increased by at least 11% and 25% in the presence of TWEEN-40 and blends of TWEENs, respectively. Precipitation of invertase with acetone was neither affected by HLB nor by surfactant type and concentration. However, HLB and total invertase activity were linearly correlated, indicating that in an emulsified solution, such as aqueous two-phase systems, the use of surfactants could be more effective on the precipitation of invertase with acetone.
Authors and Affiliations
Michele Vitolo
Keywords
Related Articles
- EP ID EP673292
- DOI -
- Views 231
- Downloads 0
ACHYRANTHES ASPERA -A PROMISING MEDICINAL HERB
Achyranthes aspera Linn. (Amaranthaceae family) is an important medicinal plant is found throughout Bangladesh as an annual herb. The medicinal plants are used for treatment of various diseases because of their safety an...
EVALUATION OF NITRIC OXIDE RADICAL SCAVENGING ACTIVITY OF A HEALTH SUPPLEMENT, HERBALONE
Nitric oxide (NO) plays a key role in the production of reactive nitrogen species (RNS). The objectives of this study were to determine antioxidant activities of ethanol extracts of raw materials and product samples were...
PYRUVATE KINASE ISOFORM M2 AS A DIAGNOSTIC TOOL FOR RENAL CELL CARCINOMA
Renal cell carcinoma (RCC) is the most common cancer in the kidney. Pyruvate kinase is an enzyme that catalyzes the conversion of phosphoenol pyruvate and ADP to pyruvate and ATP in glycolysis. Aim of the study: The curr...
A LITERATURE REVIEW ON HYPERTENSIVE CRISIS
Hypertension (HTN) is a leading cause of global disease, mortality, and disability. In developed countries because of increased awareness regarding the health and widespread availability of antihypertensive drug, there h...
REJECT-REPEAT ANALYSIS OF PLAIN RADIOGRAPHS AS A QUALITY INDICATOR AT UNIVERSITY OF MAIDUGURI TEACHING HOSPITAL (UMTH)
Objective: To determine the level of improvement of reject rate, persistent main cause of repeated films and re-occurring most common projection(s). Methodology: A prospective design was carried out on radiographs of poo...