DETECTION OF BIOFILM PRODUCTION IN BLOOD CULTURE ISOLATES OF STAPHYLOCOCCI
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2015, Vol 4, Issue 1
Abstract
Background: Biofilm producing bacteria which are inherently resistant to antibiotics and disinfectants are widely associated with implant associated infections. Staphylococcus is the most commonly associated pathogens with bloodstream infection. Aims: The current study was conducted to detect biofilm production in Staphylococci isolated from blood culture specimens. Materials and Methods: 70 clinically significant staphylococcal isolates from blood culture were screened for biofilm production by Tissue culture plate (TCP) method, Tube method (TM) and Congo red agar (CRA) method and their antibiotic susceptibility profile was studied. Results: 59 out of 70 staphylococcal isolates were positive by TCP, out of these 21.4% staphylococci were high biofilm producers, 62.8% staphylococci were moderate biofilm producers and 15.8% were non-biofilm producers. Maximum resistance was observed in biofilm producers to cotrimoxazole (74.5%) and erythromycin (62.7%) and none were resistant to vancomycin and linezolid. Out of total 59 biofilm producers, 20.3 % (12) were methicillin resistant and all these were S. aureus isolates. 19% (1) out of total 11 biofilm non-producers were methicillin resistant. Conclusion: Biofilm production was seen to be a major virulence factor in most of the staphylococcal isolates obtained from patients with signs and symptoms of septicaemia. S. aureus was found to be the major pathogen and timely detection of biofilm producing phenotype should be carried out using a simple and reproducible method, TCP which is both qualitative and quantitative.
Authors and Affiliations
Gupta Puja| Postgraduate student, Professor & Head Department of Microbiology, HIMS, Dehradun, Uttarakhand, India, Gupta Pratima| Professor and Head, Department of Microbiology, AIIMS, Rishikesh, Uttarakhand, India, Mittal Garima| Assistant Professor, 4Professor & Head Department of Microbiology, HIMS, Dehradun, Uttarakhand, India, Corresponding author email: garimamittal80@gmail.com, Agarwal RK| Professor & Head Department of Microbiology, HIMS, Dehradun, Uttarakhand, India, Goyal Rohit| Assistant Professor, Department of Anaesthesiology, HIMS, Dehradun, Uttarakhand, India
Keywords
Related Articles
- EP ID EP11338
- DOI 10.5958/2319-5886.2015.00004.1
- Views 308
- Downloads 11
Influence of physical exercise on interleukin-17, cortisol and melatonin levels in serum, whole blood and mitogen activated peripheral blood mononuclear cells
The purpose of this study was to assay the effect of two months exercise and two months silent on the levels of interleukin-17 (IL-17), melatonin and cortisol in serum, whole blood (WB) and peripheral blood mononuclear...
Te Relation Between Laparoscopic Sleeve Gastrectomy and Ghrelin
Recently, obesity has become an important worldwide health problem. One of the obesity treatment alternatives is bariatric surgery methods and their effciency is increasing from day to day. In laparoscopic Sleeve gastre...
Development of Verbal Expressive Skills Management Programme (VESMP) for Patients with Broca’s Aphasia
Introduction: Disorders of communication, including aphasia (mainly post stroke) caused by the left hemisphere brain damage, is a major community health issue. The prevalence of aphasia after stroke is 25% in Pakistan i...
CLINICAL PATTERN AND EFFECT OF CO-MORBIDITIES IN THE ETIOPATHOGENESIS OF INCISIONAL HERNIAS
Background: Incisional hernia is a common iatrogenic complication of abdominal surgery and is a cause of unwanted morbidity. The study was reported for the first time from Republic of Mauritius. Aims & Objectives: The...
RARE PRESENTATION OF RUPTURED RUDIMENTARY HORN PREGNANCY
It is a rare occurrence for the rudimentary horn of uterus to harbour a pregnancy and the usual outcome is devastating leading to a spontaneous rupture in second trimester with the patient presenting in shock with mas...