Effect of Lead Stress on Chlorophyll Content, Malondialdehyde and peroxidase activity in Seedlings of mung bean (Vigna radiata)
Journal Title: International Journal of Research in Chemistry and Environment (IJRCE) - Year 2013, Vol 3, Issue 3
Abstract
The presence of heavy metals in solid and liquid wastes is an important issue that is related to the pollution of the environment. These are one of the most important environmental pollutants and reaching dangerous amount requires more research. Among the heavy metals, lead particularly has become a cosmopolitan enviornmental pollutant. This study was conducted to investigate the effects of Pb stress on different varieties (Virat, SML-668, A1 Gold and K-851) of mung bean (Vigna radiata). V.radiata occupies a very important position in Indian agriculture. Seeds of four different mung bean varieties were grown under laboratory conditions in dark at 0- 1500 μM concentration of lead nitrate [Pb(NO3)2]. Distilled water was used as control treatment. To access the effect of lead stress on peroxidase activity, Malondialdehyde (MDA) and chlorophyll content,7 day old seedlings were used. The results revealed that the chlorophyll content of of mung bean seedlings significantly (p<0.05) decreased with increasing concentrations of [Pb(NO3)2]. MDA and peroxidase activity were significantly (p<0.05) increased proportionately (r > 0.9) to the increasing concentration of [Pb(NO3)2]. Increased amount of MDA was indicative for the formation of free radicals in plants under heavy metal stress, while increased level of peroxidase activity pointed to the occurrence of a scavenging mechanism. The decrease in chlorophyll content is an indication of reduction in the growth of the plants leading to a decrease in the yield.
Authors and Affiliations
Dugar Deshna, Bafna A.
Keywords
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