Effects of electroacupuncture at auricular concha area on the circadian rhythm of melatonin secretion in p-chlorophenylalanine-induced insomnia rats
Journal Title: TMR Non-Drug Therapy - Year 2020, Vol 3, Issue 1
Abstract
Backgroud: The electroacupuncture of auricular concha area (EA-ACR) proved to have a good regulatory effect on insomnia and the release of melatonin, which is most closely related to sleep. However, there is little research to demonstrate the relationship between EA-ACR and melatonin. The objective of present study is to observe the effects of EA-ACR on the circadian rhythm of melatonin secretion in p-chlorophenylalanine (PCPA)-induced insomnia rats. Methods: The study divided 36 male Sprague-Dawley rats randomly into three groups: 12 in blank group, 12 in model group, and 12 in EA-ACR group. By using PCPA intraperitoneal injection for 2 days, insomnia model was established. EA-ACR group was treated for 28 days. We observed the effects of EA-ACR on the rats' behavioral changes, open-field test and weight during the experiment. After the experiment, the rat tail and heart blood were collected, and the melatonin was detected by enzyme-linked immunosorbent assay. Results: Compared with the blank group, 28–30 hours after PCPA intraperitoneal injection, the circadian rhythm of sleep and the total sleep time of model rats decreased. Compared with the model group, electrical stimulation in the ear concha region could effectively reverse the behavioral changes of rats, and increase the trend of weight growth (P = 0.010), the crossing score (P = 0.014), vertical motion score (P = 0.015) and the level of plasma melatonin secretion (P = 0.020 ) with the trend showing a rhythmic pattern (0–60 minutes upward , 60–120 minutes downward, 120–150 minutes upward). Conclusion: EA-ACR can improve the quality of life (weight), ameliorate exploring irritable and anxiety behaviors in PCPA-induced insomnia rats. The underlying effects of EA-ACR may be excited on the circadian rhythm of melatonin secretion in PCPA-induced insomnia rats.
Authors and Affiliations
Wen-Ya Huang, Wei-Wei Xu, An-Ning Zhu, Si-Yu Liu, Yang Huang, Peng Li, Zong-Bao Yang, Shu-Qiong Huang, Xian-Jun Meng
Keywords
Related Articles
- EP ID EP678601
- DOI 10.12032/TMRND20200121001
- Views 182
- Downloads 0
Dodick DW. Migraine. Lancet. 2018;391(10127): 1315–1330.
Background: Cupping therapy is one of integrative medicine modalities which is used by several nations. Its mechanism of action is not well known but there are several theories explained how it can play a rule in the tre...
Comparison of horse and cow milk on sleep disorders in children with attention deficit hyperactivity disorder: a double-blind randomized clinical trial study
Approximately 25%–50% of children with attention deficit hyperactivity disorder have sleep problems. The aim of this study is to compare the effect of horse milk with conventionally-fed cow’s milk on sleep disorders in c...
Effect of acupuncture on short-term pain after standard thoracotomy
Objective: To observe the effect of perioperative acupuncture on postoperative pain in patients undergoing standard sternotomy. Methods: 57 patients who received standard median sternotomy were randomly divided into acup...
Research progress of acupuncture treatment on postpartum depression in recent ten years
In recent years, postpartum depression has gained increasing attention around the world due to its high prevalence and severe health consequences. Meanwhile, a considerable number of postpartum depression patients seek h...
Acupuncture combined with Buyang Huanwu decoction in the treatment of sequelae of cerebrovascular accident: a systematic review and meta-analysis
Background: Cerebrovascular accident has high morbidity, high disability, and high mortality, possibly causing several serious sequelae and bringing a heavy burden to the patient, the family, and the society. Buyang Huan...