SYNTHETIC ANTI-MELAMINE RECEPTOR BASED ON MOLECULARLY IMPRINTED POLYMERS FOR PSEUDO-ELISA
Journal Title: Сенсорна електроніка і мікросистемні технології - Year 2015, Vol 12, Issue 2
Abstract
It is shown the possibility of using synthetic receptors (based on solid-phase mo-lecular imprinting) as a cheaper alternative to antibodies during the ELISA detection of mela-mine. Previous quantum-chemical calculations allowed to justify the choice (among a mini-library of 13 monomers) of the best functional monomer, namely, acrylamido-2-methyl-1-propanesulfonic acid to create "artificial receptor" on the melamine based on molecular imprinting technology. The detection limit for melamine using competitive method was 0.0058 nm (5.8 pM), the linear concentration range: 0.01 – 1 nM. The sensitivity of this method of melamine detection three times above than the ELISA analysis using antibodies.
Authors and Affiliations
K. M. Мuzyka, M. M. Rozhitskii
Keywords
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- EP ID EP399192
- DOI 10.18524/1815-7459.2015.2.73667
- Views 86
- Downloads 0
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