The antioxidant activity of BHT and new phenolic compounds PYA and PPA measuredby chemiluminescence.
Journal Title: Cellular and Molecular Biology Letters - Year 2001, Vol 6, Issue 1
Abstract
The antioxidative properties of two series of new phenolic, amphiphiliccompounds were evaluated using the chemiluminescence (CL) method. 2,2'-Azobis (2-amidinopropane) dihydrochloride(AAPH) was used as a source of free radicals, to obtain high and prolonged CL. Three different kindsof buffers (organic and inorganic) were tested. The CL level varied only slightly depending on the bufferbut increased significantly with the pH. Twelve newly synthesised compounds were compared with butylatedhydroxytoluene (BHT), a commercially used antioxidant. The new antioxidants included two classes of quaternaryammonium salts with a phenol substituent functioning as an antioxidant. The salts were synthesised byquaternarization of pyrrolidine ethyl esters of dihydrocinnamic acid by n-alkoxymethyl bromides (PYA-n)or quaternarization of 2-dimethylaminoethyl esters by n-alkyl bromides (PPA-n). All the tested compoundsquenched CL proportionally to their concentrations. In our experimental conditions 8.5 microM BHT quenched50% of the CL. The PYA and PPA compounds had IC50 two to six times lower than BHT. CL inhibition wasproportional to the pH for all antioxidants. The relationships between the structure and activity ofthe tested compounds are discussed.
Authors and Affiliations
A Krasowska, D Rosiak, K Szkapiak, M Oświecimska, S Witek
Keywords
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