THE LEVEL OF DIFFERENT FORMS OF HEMOGLOBIN IN THE HEMOLISAT OF THE ERYTHROCYTES OF RATS BLOOD ON CONDITION OF THE LACK OF PROTEIN
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2014, Vol 6, Issue 2
Abstract
In this work has been researched the general level of hemoglobin and its main derivative - oxyhemoglobin in hemolisates of the rats' erythrocytes under conditions of nutritional deprivation of protein. At the same time considerable attention was paid to the determination of glycosylated hemoglobin content - marker of disruptions of carbohydrate metabolism in the body under conditions of protein deficiency. In order to study the characteristics of these biochemical indicators under conditions of nutritional protein deprivation experimental animals were kept for 28 days on a semi-synthetic low-protein diet on the principle of pair food. Hemolisate of erythrocytes was obtained by Drabkin method with subsequent removal of the reticular stroma. Determination of total hemoglobin was made by a hemoglobincyanide method, oxyhemoglobin - by a spectrophotometric method by means of the molar extinction coefficient at achieving the maximum level of oxygenation of the molecule. The level of glycosylated hemoglobin in red blood cells' hemolisate was determined by reaction with the thiobarbituric aicid. It was found out that under conditions of protein deficiency in rats' blood hemolisate is a decrease in the concentration of total hemoglobin and oxyhemoglobin 1,4 times compared to the control indicators. It should be noted that the decrease of hemoglobin under conditions of protein deficiency on the one hand, it may be due to impaired synthesis of globin polypeptide chains due to a deficiency of aminoacids, on the other hand - a disruption of heme synthesis. At the same time in the experimental group of animals has been observed the decrease of main ligand form of hemoglobin - oxyhemoglobin, which may indicate the disruption of binding and kickback of oxygen and the resulting changes in the affinity of hemoglobin for oxygen. At the same time we studied that hemolisate of red blood cells of rats who were on a low-protein diet has double increase in glycosylated hemoglobin compared to control indicators, which is considered as a prognostic indicator of metabolic hyperglycemia, because it reflacts percentage of hemoglobin, irrevocably conncted to glycose. Glycosylated hemoglobin due to a strong relationship with oxygen gives it hard to tissues, so increasing of its content in the blood causes the occurrence of tissue hypoxia, which is related to insufficient oxygenation of basal membrans.
Authors and Affiliations
Г. П. КОПИЛЬЧУК, І. М. БУЧКОВСЬКА, М. Г. СКРИПНИК
Keywords
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