结缔组织生长因子反义寡核苷酸体外对人增生性瘢痕成纤维细胞凋亡的影响

Journal Title: Di-er Junyi Daxue Xuebao - Year 2007, Vol 28, Issue 8

Abstract

目的:观察结缔组织生长因子(CTGF)反义寡核苷酸(ASODN)体外对人增生性瘢痕组织中成纤维细胞凋亡的影响并探讨其可能的作用机制。方法:体外培养的人增生性瘢痕组织中成纤维细胞, 分为CTGF-ASODN 脂质体治疗组(ASODN treatment, AT)、空白脂质体对照组(liposome control,LC) 和空白对照组(control,C),荧光显微镜观察各组CTGF-ASODN时相性分布,流式细胞术检测各组凋亡率随时相的变化,观察Fas受体表达;RT-PCR检测各组CTGF、Fas、 bcl-2 mRNA的表达。结果:AT组细胞转染24 h 后胞质内可见有大量黄绿色荧光,呈离散型、点状分布,而LC 和C 组成纤维细胞内未见荧光。转染24 h后LC组和C组成纤维细胞的凋亡率及Fas受体阳性表达率无显著差异;而AT组凋亡率及Fas受体阳性表达率明显增加,明显高于LC组和C组(P<0.01)。RT-PCR结果表明,转染组CTGF mRNA相对表达量明显低于空白对照组(P<0.05),Fas mRNA表达明显高于空白对照组(P<0.01), 转染组bcl-2 mRNA与空白对照组无显著差异。结论:CTGF反义寡核苷酸体外能促进人增生性瘢痕成纤维细胞凋亡,可能与其上调Fas mRNA及其蛋白表达有关。

Authors and Affiliations

Ming XU, Xin XING

Keywords

Related Articles

Optimized design for in vitro experiment of electret transdermal patches

Objective:Currently most researches concerning electret transdermal patches are performed by one-factor experimental design with relatively poor efficiency. To analyze the influencing factors in a more systematical and e...

Expression of DNA methyltransferase 1 and histone deacetylase 1 proteins in intraductal papillary mucinous neoplasms

Objective:To investigate the expression of DNA methylation transferase 1 (DNMT1) and histone deacetylase 1 (HDAC1) proteins in intraductal papillary mucinous neoplasms (IMPNs) and its possible clinical significance.Metho...

99mTc-MIBI myocardial perfusion image in predicting therapeutic effect of percutaneous coronary intervention

Objective:To observe the 99mTc-MIBI myocardial perfusion images in patients with myocardial infarction before/after percutaneous coronary intervention (PCI),so as to screen for the reliable predictors for the therapeuti...

人格、家庭因素与军人焦虑情绪的相关性研究

目的:研究军人人格特质、家庭因素与焦虑的关系,分析军人焦虑的影响因素,为建立有效的干预措施提供理论依据。方法:应用艾森克人格问卷(EPQ)、状态-特质焦虑问卷(STAI)及自拟的一般情况调查表对2 001名男性军人进行团体心理测试,分析...

熵指数在老年患者七氟醚或丙泊酚靶控输注诱导中的应用

目的:探讨熵指数在老年患者七氟醚吸入或丙泊酚靶控输注诱导时的应用价值。方法:拟行气管插管全麻的老年患者40例,ASA Ⅰ~Ⅱ级,年龄≥65岁,随机分为七氟醚组(S组)和丙泊酚组(P组),分别以七氟醚吸入和丙泊酚靶控输注诱导,诱导期间调节熵指数维...

Download PDF file
  • EP ID EP83996
  • DOI -
  • Views 145
  • Downloads 0

How To Cite

Ming XU, Xin XING (2007). 结缔组织生长因子反义寡核苷酸体外对人增生性瘢痕成纤维细胞凋亡的影响. Di-er Junyi Daxue Xuebao, 28(8), 822-826. https://www.europub.co.uk/articles/-A-83996