A Comparative Study Of Agnor With Pcna Immunohistochemical Staining In Oral Squamous Cell Carcinoma
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH - Year 2017, Vol 9, Issue 5
Abstract
Background: The present study was to compare Argyrophilic Nucleolar Organizer Regions (AgNORs) with Proliferating Cell Nuclear Antigen (PCNA) expression in Oral squamous cell carcinoma (OSCC) and to assess the proliferating activity in OSCC, also to assess the reliability between PCNA and AgNOR. Methods: Previously confirmed 30 cases of oral squamous cell carcinomas were taken for the study. Histological sections were prepared from paraffin embedded blocks and processed for AgNOR stain and PCNA stain according to super sensitive polymer HRP method. The statistical evaluation by ANOVA test helped us to compare and correlate between the two methods. Results: PCNA expression in Well-differentiated OSCC was 61.6471% followed by moderately differentiated OSCC with 72.4% and poorly differentiated OSCC with 89%. The AgNOR count in the well differentiated OSCC had a mean value of 3.4 followed by moderately differentiated OSCC with 3.9 and poorly differentiated OSCC with 9.4. In the current study, on correlation between PCNA and AgNOR, the pearsons co-relation coefficient value was found to be highly significant. Whereas the relation between PCNA and AgNOR within the grades of OSCC were found to be not correlated. This was statistically not significant. Conclusions: Proliferation activity was more in poorly differentiated OSCC followed by moderately differentiated and well differentiated OSCC. The combination of PCNA immunohistochemistry with AgNOR count appears to be an effective means of identifying the proliferating activity of squamous cell carcinoma, thereby appropriate treatment can be given.
Authors and Affiliations
Samir Ahmed, Dinkar Desai, Pramod Redder Chandrappa, Siddarth Pandit, Sharan. Jayram. Shetty, Dr. Suresh, K. V .
Keywords
Related Articles
- EP ID EP403816
- DOI -
- Views 121
- Downloads 0
Knowledge And Practices Of Adolescent Girls Regarding Mensturation In Government Girls High School, Kadapa, Andhra Pradesh
Introduction: Menstruation is the first step of a girl to start her reproductive life. Menstruation is a unique phenomenon to the female. The onset of menstruation is one of the important changes occurring among the girl...
Prevalence of respiratory tract infections in children under five years at Quetta
Objectives: To study the prevalence of respiratory tract infections in children under five years. Background: Respiratory tract infection is an important cause of morbidity and mortality in children under five years of a...
Fournier’s gangrene: Our experience with 50 patients and analysis of factors affecting mortality
Fournier’s gangrene (FG) is a rare, rapidly progressive, fulminant form of necrotizing fasciitis of the genital, perianal and perineal regions, which may extend up to the abdominal wall between the fascial planes. Early...
The anthropometric measurements in full term neonates in Baghdad
Determination of newborn growth parameters are necessary in each population for planning their subsequent children growth charts and early detection of diseases. To determine the normal standards of anthropometric measu...
Barriers of patient health teaching among nursing students
Background: Nursing literature indicates that patient teaching is a critical component of nursing roles, and health teaching is of the basic rights of the patients. Nursing students facing challenges and constraints whil...