A comparative study of in vitro contractility between gut tissues of Hirschsprung’s disease and other gut malformations
Journal Title: National Journal of Physiology, Pharmacy and Pharmacology - Year 2017, Vol 7, Issue 11
Abstract
Background: Hirschsprung’s disease and other gut malformations commonly present with obstructive features of gut in pediatric age group. Problem of obstruction persists even after resection and anastomosis operation. Aims and Objectives: This in vitro comparative study was performed to assess the gut contractility to chemical mediators such as Acetylcholine and Histamine between Hirschsprung’s disease and other gut malformations. Materials and Methods: The longitudinal muscle strips of Hirschsprung’s cases and other gut malformations (non-Hirschsprung’s cases) were placed in Dales organ bath containing Krebs-Ringer solution, continuously bobbled with 100% O2 at 28°C. Gut contractions were recorded using Power Lab 4/ST system and was analyzed using software CHART-5 for windows. Control contractions were recorded against initial tension of 0.5 g. Subsequently, agonist (acetylcholine, histamine)- induced contractions were recorded before and after appropriate antagonists (atropine, pheniramine). Before values of agonist-induced contractions of Hirschsprung and non-Hirschsprung’s cases were compared. Values of agonistinduced contractions obtained after pretreatment with antagonists were also compared between Hirschsprung and nonHirschsprung’s cases. Results: Acetylcholine enhanced contractions in non-Hirschsprung’s cases and it caused small increase in amplitude of contractions in Hirschsprung’s cases. Atropine pretreatment blocked acetylcholine-induced contractions significantly in non-Hirschsprung’s cases, whereas it failed to block in the Hirschsprung’s cases. Histamine augmented contractions in both Hirschsprung and non-Hirschsprung’s cases. H1 antagonist, pheniramine failed to block the contractility in both the cases. Conclusion: Findings of this study suggested that acetylcholine increased gut contractility significantly in non-Hirschsprung’s cases involving muscarinic-cholinergic pathways, whereas histamine increased gut contractility in both Hirschsprung’s disease and non-Hirschsprung’s cases and it is not mediated by H1 receptors.
Authors and Affiliations
Anil Kumar Tiwari, Sanjeev K Singh, Ratna Pandey, Phani Bhushan Singh, Shashikant C U Patne, Ajay N Gangopadhyay, Maloy B Mandal
Keywords
Related Articles
- EP ID EP292104
- DOI 10.5455/njppp.2017.7.0623205072017
- Views 91
- Downloads 0
The anxiolytic effect of bee pollen hydroalcoholic extract in mice
Background: Anxiety is a common human mental disorder. Medicinal therapy of this disease is associated with many sideeffects. Thus, search for new medication with fewer side-effects seems inevitable. Bee pollen (often re...
A survey on technical approaches in fall detection system
The fall events have become a common health problem among elderly people. The accidental falls are a serious issue. If it is unnoticed, then it becomes fatal. The concept of automatic fall detection technique is m...
Study of drugs used in cerebral edema in patients with stroke at a tertiary care center
Background: Various medical and surgical measures have been evolved to treat the raised intracranial pressure (ICP). Medical management mainly involves osmotic agents (glycerol and mannitol), diuretics, and corticosteroi...
Risk factors for pneumonia in acute stroke patients admitted to the Emergency Department of a Tertiary Government Hospital
Background: Knowledge on the risk factors of developing pneumonia, one of the top causes of mortality in acute stroke patients admitted to a tertiary government hospital, is lacking. Aim and Objective: This study aimed t...
“TRAIT” level of perceived anxiety and effect of instrumental background music after induced stress test on “STATE” level of perceived anxiety in students from different ethnic groups - A randomized control trial
Background: Stress in medical students is well known, but ethnic variations in general-anxiety levels and effect of instrumental music, in relation to stress test, on anxiety in these ethnicities remain obscure. Aims and...