A comparative study of in vitro contractility between gut tissues of Hirschsprung’s disease and other gut malformations
Journal Title: National Journal of Physiology, Pharmacy and Pharmacology - Year 2017, Vol 7, Issue 11
Abstract
Background: Hirschsprung’s disease and other gut malformations commonly present with obstructive features of gut in pediatric age group. Problem of obstruction persists even after resection and anastomosis operation. Aims and Objectives: This in vitro comparative study was performed to assess the gut contractility to chemical mediators such as Acetylcholine and Histamine between Hirschsprung’s disease and other gut malformations. Materials and Methods: The longitudinal muscle strips of Hirschsprung’s cases and other gut malformations (non-Hirschsprung’s cases) were placed in Dales organ bath containing Krebs-Ringer solution, continuously bobbled with 100% O2 at 28°C. Gut contractions were recorded using Power Lab 4/ST system and was analyzed using software CHART-5 for windows. Control contractions were recorded against initial tension of 0.5 g. Subsequently, agonist (acetylcholine, histamine)- induced contractions were recorded before and after appropriate antagonists (atropine, pheniramine). Before values of agonist-induced contractions of Hirschsprung and non-Hirschsprung’s cases were compared. Values of agonistinduced contractions obtained after pretreatment with antagonists were also compared between Hirschsprung and nonHirschsprung’s cases. Results: Acetylcholine enhanced contractions in non-Hirschsprung’s cases and it caused small increase in amplitude of contractions in Hirschsprung’s cases. Atropine pretreatment blocked acetylcholine-induced contractions significantly in non-Hirschsprung’s cases, whereas it failed to block in the Hirschsprung’s cases. Histamine augmented contractions in both Hirschsprung and non-Hirschsprung’s cases. H1 antagonist, pheniramine failed to block the contractility in both the cases. Conclusion: Findings of this study suggested that acetylcholine increased gut contractility significantly in non-Hirschsprung’s cases involving muscarinic-cholinergic pathways, whereas histamine increased gut contractility in both Hirschsprung’s disease and non-Hirschsprung’s cases and it is not mediated by H1 receptors.
Authors and Affiliations
Anil Kumar Tiwari, Sanjeev K Singh, Ratna Pandey, Phani Bhushan Singh, Shashikant C U Patne, Ajay N Gangopadhyay, Maloy B Mandal
Keywords
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- EP ID EP292104
- DOI 10.5455/njppp.2017.7.0623205072017
- Views 92
- Downloads 0
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