A Cross Sectional Study to Assess the Blood Stream Infection Amongst the Neonates, Infants And Children in A Tertiary Care Set Up of Eastern India
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 12
Abstract
Blood culture remains the mainstay of laboratory diagnosis of bloodstream infections in infants and children and neonatal septicaemia. The cross sectional study was performed with 96 cases over a period of three months from 15th October to 14th December, 2017 in a tertiary care set up. Aerobic and anaerobic blood culture for clinically suspected cases was performed by automation (BACTEC). Blood volume and number samples were decided as per standard protocols. Blood culture bottles with positive growth indicated by automation were subcultures on Mac conkey’s agar media, blood agar media and chocolate agar media. Antibiotic susceptibility testing was done as per standard microbiological guidelines by Kirby Bauer’s technique. A total of 96 blood culture (88 aerobic and 8 anaerobic) was performed out of which, in 25 cases, bacterial growth was obtained (26.04%). Out of these 25 cases, in one case, anaerobic culture indicated presence of bacterial growth. Amongst 24 aerobic growth, 13 cases were due to Staphylococcus aureus, 5 cases were due to Salmonella Typhi, 3 was Escherichia coli, Klebsiella pneumoniae was isolated in 2cases and in one case, blood stream infection was caused by Staphylococcus haemolyticus. Streptococcus pneumonia was isolated in the case where blood culture showed growth in anaerobic condition. Escherichia coli was found to be the major Gram negative pathogen to cause neonatal septicaemia (28%). Isolated Streptococcus pneumoniae was sensitive to Penicillin, Ampicillin, Amoxycillin- Clavulanic acid, Erythromycin, all four generations of Cephalosporins, Vancomycin, Linezolid and Teicoplanin. 18 of the isolated 24 aerobic bacteria were resistant to fluoroquinolones (75%).
Authors and Affiliations
Dr. Sanjeev Das, Dr. Jayashree Konar, Mr. Asim Kumar Pahari
Keywords
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- EP ID EP251499
- DOI 10.9790/0853-1612104648
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