A New Protocol for Collagen-Induced Local Arthritis Model in Balb/c Mice
Journal Title: Turkish Journal of Immunology - Year 2018, Vol 6, Issue 1
Abstract
Introduction: Numerous models have been described to study the pathogenesis of rheumatoid arthritis, and to develop new therapies; but each of these models has their own limitations. Nowadays, the collagen-induced arthritis (CIA) mouse model is the most commonly used model for rheumatoid arthritis studies in certain mouse strain like DBA/1 mouse. This study aimed to describe a new protocol for local induction of arthritis in Balb/c mice, including the monitoring of clinical arthritis and the protocols for histological examination of paws of mice. Materials and Methods: For the induction of local arthritis in 40 Balb/c mice, they were immunized intra-articularly with type II collagen and subcutaneous complete Freund’s adjuvant in 0 day. As the boost immunization, the animals had same injections on day 14. Mice were divided 4 groups, and they were clinically and histopathologically examined for arthritis on 0, 14, 21 and 30 days of arthritis induction. Results: The first signs of local arthritis appeared in this model 1 week after boost immunization (day 21). The CIA induced paw clinically showed severe erythema and swelling all around the hindquarter on day 21 and 30. The paw reached almost 4 times thicker than the other paws and histopathological examination confirmed the clinical arthritis on day 21 and 30. Conclusion: Using the protocol described, the investigators may reproducibly and economically induce a high incidence of local CIA in Balb/c mice. The described local CIA model may be used to unravel pathophysiological or immunological mechanisms of arthritis, and can also be used to study the effect of new therapeutics. Keywords: Experimental model, rheumatoid arthritis, collagen-induced arthritis, Balb/c mouse
Authors and Affiliations
Gökçen Güvenç, Mehmet Karaçay, Ahmet Akkoç, Kubra Çiftçi, Haluk Barbaros Oral, Murat Yalçın
Keywords
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- EP ID EP450692
- DOI 10.25002/tji.2018.771
- Views 86
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