A NOVEL VALIDATED RP-HPLC METHOD FOR THE DETERMINATION OF ITOPRIDE HYDROCHLORIDE IN BULK AND PHARMACEUTICAL TABLET DOSAGE FORMS  

Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 4

Abstract

A simple, specific, accurate, rapid, inexpensive isocratic Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for the quantitative determination of Itopride HCl in pharmaceutical tablet dosage forms. RP-HPLC method was developed by using Welchrom C18Column (4.6 X 250mm, 5µm), Shimadzu LC-20AT Prominence Liquid Chromatograph. The mobile phase composed of 10mM Phosphate buffer (pH-3.0, adjusted with triethylamine): acetonitrile (50:50v/v). The flow rate was set to 1.0 mL.min-1 with the responses measured at 235nm using Shimadzu SPD-20A Prominence UV-Vis detector. The retention time of Itopride HCl was found to be 2.650 min. Linearity was established for Itopride HCl in the range of 2-10 µg.mL-1 with correlation coefficient 0.9999. The validation of the developed method was carried out for specificity, linearity, precision, accuracy, robustness, limit of detection, limit of quantitation. The developed method can be used for routine quality control analysis of Itopride HCl in pharmaceutical tablet dosage form. 

Authors and Affiliations

P. Ravisankar, G. Rao, M. Chaitanya, Ch. Devadasu

Keywords

Related Articles

USE OF MIFEPRISTONE AND MISOPROSTOL COMBINATION ON ABORTION IN FIRST TRIMESTER PREGNANCY AT GOVERNMENT HOSPITAL IN CHITWAN DISTRICT IN NEPAL  

The present study is a prospective study and was aimed to identify the type and pattern of side effects during and after a medical abortion using a combination therapy of Mifepristone and Misoprostol, association between...

EVALUATION OF ZINGIBER OFFICINALE AND CURCUMA LONGA RHIZOME AS A CRUDE DRUG FROM THEIR ETHANOLIC EXTRACT  

Zingiber officinale and Curcuma longa has a lot of potential in terms of medicinal properties. Both crude drug rhizomes comprises of the same family, Zingiberaceae. Plants based medicaments are the sources for modern pha...

EFFECT OF SHORT TERM EXPOSURE OF BUTYLPARABEN ON UTERINE WEIGHT OF OVARIECTOMISED C3H ALBINO MICE 

Butylparaben is a commonly used anti microbial preservative in cosmetics, pharmaceuticals and food. The high rate of human exposure of butylparaben has been of growing concern as it is found to mimic estrogen activity in...

MEDICINAL PLANTS AGAINST LIVER DISEASES 

India is the largest producer of medicinal plants and is rightly called the “Botanical Garden of the World”. The medicinal plants have very important place in the health and vitality of human beings as well as animals. A...

EVALUATION OF NIMBA TAILA AND MANJISTHA CHURNA IN NON HEALING ULCER 

This clinical study was done in fifty-one cases of non-healing ulcers of more than six weeks duration to evaluate the efficacy of Nimba taila (Neem oil) externally and Manjistha churna (powder) orally as well as its kwat...

Download PDF file
  • EP ID EP135781
  • DOI 10.7897/2230-8407.04429
  • Views 106
  • Downloads 0

How To Cite

P. Ravisankar, G. Rao, M. Chaitanya, Ch. Devadasu (2013). A NOVEL VALIDATED RP-HPLC METHOD FOR THE DETERMINATION OF ITOPRIDE HYDROCHLORIDE IN BULK AND PHARMACEUTICAL TABLET DOSAGE FORMS  . International Research Journal of Pharmacy (IRJP), 4(4), 145-151. https://www.europub.co.uk/articles/-A-135781