A validated analytical method for the estimation of Oxetacaine from its pharmaceutical formulation by RP-HPLC
Journal Title: International Journal of Research and Development in Pharmacy & Life Sciences (IJRDPL) - Year 2017, Vol 6, Issue 5
Abstract
OBJECTIVE: The aim of the current study was to develop an analytical procedure for the determination and quantification of Oxetacaine, from its marketed formulation which is simple, precise, accurate and a validated, reverse phase high performance liquid chromatography (RP-HPLC) method. METHOD: The optimized chromatographic condition was achieved on a kromasil C18 (150 X 4.6 mm i.d., 5µ) as stationary phase and Acetonitrile: 10mM Potassium dihydrogen-ortho-phosphate (pH 3.5) in the ratio 30:70 % v/v as mobile phase, with a flow rate of 1.0 ml/min. The detector response for the method was determined, and the quantification was carried out at 214 nm. RESULT: Oxetacaine was eluted at 6.0 min. The quantification was performed using calibration curve method, and the linearity was achieved from 10-50 µg/ml. The percentage recovery was found to be 98.0 ± 1.20 respectively. The correlation coefficient was found to be 0.9954 respectively. The limit of detection (LOD) and limit of quantification (LOQ) for the current method was achieved at 1µg/mL and 5µg/mL respectively. CONCLUSION: The current study was performed at ambient temperature, and the method is simple, selective, linear, precise, accurate and sensitive which can be used for the routine analysis of Oxetacaine tablets. The developed method was validated as per ICH guidelines.
Authors and Affiliations
S. T. Narenderan| Department of Pharmaceutical Analysis, B. Babu| Department of Pharmaceutical Analysis, S. N. Meyyanthan| Department of Pharmaceutical Analysis, B. Gowramma| Department of Pharmaceutical Chemistry,JSS College of Pharmacy (A Constituent College of Jagadguru Sri Shivarathreeswara University, Mysore), Udhagamandalam, India
Keywords
Related Articles
- EP ID EP16037
- DOI http://dx.doi.org/10.21276/IJRDPL.2278-0238.2017.6(5).2764-2
- Views 419
- Downloads 18
EVALUATION OF ANTI-INFLAMMATORY POTENTIAL OF ETHANOLIC EXTRACT OF THE LEAVES OF RHIZOPHORA MUCRONATA, A SUNDERBAN MANGROVE
Objective: Biochemically unique mangrove plants are recognized worldwide for valuable medicinal actions. Experimental investigations of these pharmacological activities are needed for elucidation of cost effective new t...
Standardization of roots of Calotropisprocera and Calotropis gigantean via evaluation of morphological and physicochemical parameters
BothCalotropis giganteanand Calotropisprocera are medicinal shrub, belongs to the family Asclepiadaceae. Both plants are used in Ayurvedic system of medicine for healing various diseases. However, the present study was...
A COMPARATIVE STUDY OF BUPIVACAINE AND FENTANYL - BUPIVACAINE IN LOWER ABDOMINAL SURGERY UNDER EPIDURAL ANESTHESIA
Background: Fentanyl is an opioid general anaesthetic acting through the opioid receptors. Combination with local anaesthetics increases the onset and duration of analgesia. The present study compared the efficacy profi...
Antioxidant properties of polyphenolic rich HPLC standardized extract of Beta vulgaris L. roots
OBJECTIVES: The present study was planned to evaluate the antioxidant effects of standardized Beta vulgaris L. or beetroot. METHODS: The polyphenols present in lyophilized water extract of beetroot (BR) were quantifie...
Acute toxicity study of Mesenchymal Stromal cells derived from Wharton’s Jelly in mouse by intravenous and subcutaneous route
Aim: The present study was to evaluate the acute toxicity of WJ-MSCs in mouse by intravenous and subcutaneous route and to assess their potential for side effects, MLD, MTD and LD50. Objectives: Wide ranges of clinical...