Activation and comparative kinetics of Ovis aries plasmin with human plasmin
Journal Title: Revista MVZ Cordoba - Year 2011, Vol 16, Issue 1
Abstract
Objective. Compare the kinetics and activations, unify the purifications methods and establish the sequence of the N-terminals of the plasminogens human and Ovis aries. Materials and methods. The plasminogens were purified by the same methods: affinity and ion exchange chromatographies, furthermore both plasminogen were activated by human urokinase and the N-terminals sequence perform by the Edman’s degradation’s method. Results. The affinity of Ovis aries plasmin for the chromogenic substrate was determined in 0.45 mM, 11.8 times more affinity that the human plasmin (5.3 mM). Conclusions. The method of the plasminogens purification and the unification was established for all mammals. The high affinity of the Ovis aries plasmin confirmed a mayor affinity of animal’s plasmin to the chromogenic sustrate, compared with the human plasmin.
Authors and Affiliations
Omaira Cañas B, BSc| Universidad de Pamplona, Facultad de Ciencias Básicas, Grupo de Investigación en Química, Ciudadela universitaria, Km 1, Vía Bucaramanga, Pamplona, Norte de Santander, Colombia., Alfonso Quijano P, Ph. D| Universidad de Pamplona, Facultad de Ciencias Básicas, Grupo de Investigación en Química, Ciudadela universitaria, Km 1, Vía Bucaramanga, Pamplona, Norte de Santander, Colombia., Fernando Arbeláez R, Ph. D *| Universidad de Pamplona, Facultad de Ciencias Básicas, Grupo de Investigación en Química, Ciudadela universitaria, Km 1, Vía Bucaramanga, Pamplona, Norte de Santander, Colombia.Correspondencia: lui.ferar@hotmail.com.
Keywords
Related Articles
- EP ID EP3190
- DOI -
- Views 408
- Downloads 19
Karyotype of Espeletiopsis muiska
Objective. The cell cycle and chromosomic description of Espeletiopsis muiska was studied in meristematic cells of root tips obtained from in vitro culture of embryos on MS medium supplemented with IBA. Materials and met...
ACUTE TOXICITY AND HISTOPATHOLOGICAL LESIONS IN SILVER PACU (Piaractus brachypomus) EXPOSED TO A MIXTURE OF THE HERBICIDE ROUNDUP AND THE SURFACTANT COSMOFLUX 411F
Objective. To determine the 50% lethal concentration (LC50) and anatomopathological lesions associated with exposure to a mixture of Roundup® herbicide and Cosmoflux® 411 surfactant in juvenile silver Pacu (Piaractus bra...
ZOONOTIC TRANSMITTED BY WILD ANIMALS AND ITS IMPACT ON EMERGING AND RE-EMERGING DISEASES
Emerging and re-emerging infectious diseases originated in wild life animals can be transmitted to human population either by direct contact or by vectors. Zoonosis are of big social and epidemiological concern. The aris...
Behavior of the Hydrochoerus hydrochaeris isthmius in a productive system, department of Córdoba, Colombia
Objective. To describe the behavior, of the subspecies H. hydrochaeris isthmius, during the dry and rainy seasons in catlle farms, Valencia, Córdoba, Colombia. Materials and methods. A population of animals between Septe...
Snake report of the municipality of Tamalameque, Cesar – Colombia
Objective. Preliminary identification of the ophidians of the municipality of Tamalameque, department of Cesar Colombia. Materials and methods. Between January and May of 2009, by means of free search and manual capture...