Activation of orexin/hypocretin type 1 receptors stimulates cAMP synthesis in primary cultures of rat astrocytes.
Journal Title: Pharmacological Reports - Year 2011, Vol 63, Issue 3
Abstract
The effects of orexins, which are also named hypocretins, on cAMP formation were examined in primary cultures of rat astrocytes. Orexin A, an agonist of OX(1) and OX(2) receptors, stimulated cAMP production with an EC(50) value of 0.68 μM and potentiated the forskolin-induced increase in the nucleotide synthesis. [Ala(11)-D-Leu(15)]orexin B, an agonist of OX(2) receptors, was inactive. The effects of orexin A were antagonized by SB 408124, a selective blocker of OX(1) receptors, but were not affected by TCS OX2 29, a selective antagonist of OX(3) receptors. We hypothesized that the activation of OX(1) receptors stimulated cAMP synthesis in primary rat astrocyte cultures.
Authors and Affiliations
Agata Woldan-Tambor, Kaja Biegańska, Anna Wiktorowska-Owczarek, Jolanta Zawilska
Keywords
Related Articles
- EP ID EP108251
- DOI -
- Views 109
- Downloads 0
Peripheral antinociceptive effects of MC4 receptor antagonists in a rat model of neuropathic pain - a biochemical and behavioral study.
Recent studies have suggested that melanocortins contribute to the generation and/or maintenance of pathological pain. Experimental evidence indicates a primary role for melanocortin 4 (MC4) receptors in pathological pai...
Different effects of postnatal caffeine treatment on two pentylenetetrazole-induced seizure models persist into adulthood.
Postnatal treatment with caffeine from P7 to P11 (10 or 20 mg/kg daily) resulted in transient changes in two pentylenetetrazole (PTZ)-induced models of epileptic seizures characterized by spike-and-wave EEG rhythm in imm...
Preoperative tramadol combined with postoperative small-dose tramadol infusion after total abdominal hysterectomy: a double-blind, randomized, controlled trial.
This double blind, randomized, controlled trial investigated whether a single preoperative intravenous (iv) dose of tramadol (100 mg) given 30 min before abdominal hysterectomy resulted in improved analgesic efficacy, re...
Mangiferin ameliorates 6-hydroxydopamine-induced cytotoxicity and oxidative stress in ketamine model of schizophrenia.
Background: Accumulating evidence indicates that mangiferin (MGF), a natural xanthone, by virtue of its antioxidant and antiinflammatory properties is neuroprotective. Here we sought to verify the cytoprotective role of...
Systemic synergism between codeine and morphine in three pain models in mice.
Background: The combination of two analgesic agents offers advantages in pain treatment. Codeine and morphine analgesia is due to activation of opioid receptor subtypes. Methods: This study, performed in mice using isobo...