Active Compounds and Immunostimulating Potential of Holothuria Scabra Extract in Common Carp Fish (Cyprinus Carpio) Against Aeromonas Hydrophila Infection
Journal Title: International Journal of Zoology and Animal Biology - Year 2021, Vol 4, Issue 1
Abstract
Holothuria scabra is one of the sea cucumbers which has bioactive is assumed given beneficial effect for fish health. Therefore, this study aims to identifying of chemical compounds in ethanol H. scabra extract from coastal water in South East Sulawesi, Indonesia and to determine their potential effect as immunostimulant in common carp that were challenged using Aeromonas hydrophila infection. Immunostimulant potential test through with the administration of H. scabra extract was with immersion which doses 200 ppm, 400 ppm, 600 ppm and a treatment without the administration of H. scabra extract as positive control (K+). Then, experimental fish was challenged by A. hydrophila bacteria with cell density 107 CFU/mL, fish were injected as much as 0.1 ml/individual intra-muscularly. Result of qualitative test of active compounds indicated that ethanol extracts contained alkaloid, flavonoid and saponin. The conclusion of administration of sea cucumbers extract in different doses showed that the effect on the total erythrocyte, leukocyte and haematocrit parameters was better than the positive control but did not have a statistically significant effect. Relative Percent Survival (RPS) also showed lower survival than control fish, however fish survival among treatments did not have a statistically significant effect. Based on this, ethanol extract of H. scabra has potential as an immunostimulant in the preventive action of A. hydrophila infection, but this research needs to be continued to find out the best dosage that can be applied.
Authors and Affiliations
Azlan, Nur I* and Baytul Abidin LO
Keywords
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- EP ID EP751556
- DOI 10.23880/izab-16000271
- Views 39
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