Acute and Sub-acute Oral Toxicity Study of Drepanoalpha® (A Poly-Herbal Formula Used in the Management of Sickle Cell Disease) in Guinea-pigs
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 10, Issue 5
Abstract
Aims: To deepen the toxicological investigation of Drepanoalpha®, a poly-herbal formula used for its antisickling and radical scavenging properties in Congolese traditional medicine. Study Design: To evaluate lethality, biochemical alterations, behavioral disturbances in guinea-pigs orally given increasing doses of Drepanoalpha® aqueous extracts. Place and Duration of Study: Faculty of Medicine and Pharmacy, Official University of Bukavu, Bukavu, DR Congo, between June to December 2015. Methodology: The extracts were prepared as aqueous decoctions of Drepanoalpha powder. Animals were set to groups of 3 animals each. The doses varied from 250 mg to 16000 mg/kg body weight. Acute toxicity had to be estimated by LD50. Sub-acute toxicity was carried out by giving fractionated doses of Drepanoalpha extract during 2 weeks. Changes in blood biomarkers and behavioral signs were evaluated from baseline values of control untreated animals. Results: Up to 16000 mg/kg, no death was recorded in acute toxicity. Thus, LD50 was not calculated. No significant changes in animals behavioral were observed during sub-acute testing. Instead, body weight gain, blood cell counts increase, BUN and ALT decrease, no change in serum creatinine were noted. Conclusion: The findings comfort the safety of Drepanoalpha as found previously studies in rats.
Authors and Affiliations
P. T. Mpiana, F. M. Kasali, F. Bwirhonde, B. Z. Gbolo, D. S. T. Tshibangu, K. N. Ngbolua, P. B. Memvanga, J. N. Kadima
Keywords
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- EP ID EP342406
- DOI 10.9734/BJPR/2016/24200
- Views 114
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