ACUTE L-GLUTAMINE DEPRIVATION AFFECTS THE EXPRESSION OF G6PD, GPI, TKT, TALDO1, PGLS AND RPIA GENES IN U87 GLIOMA CELLS: EFFECT OF ERN1 KNOCKDOWN
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2014, Vol 6, Issue 1
Abstract
Pentose-phosphate cycle of glucose metabolism plays an important role in malignant tumor growth, including glioma. We have studied the expression level of mRNA of basic enzymes of this cycle (G6PD, TKT, TALDO1, PGLS and RPIA) as well as glucose-6-phosphate isomerase (GPI) in U87 glioma cells with knockdown of ERN1 (endoplasmic reticulum to nucleus signaling 1), the major sensor and signaling enzyme of endoplasmic reticulum stress, upon glutamine deprivation in the growing medium. It was shown that suppression of ERN1 signaling enzyme function leads to significant decrease of the level of TALDO1 and PGLS gene expressions in glioma cells, but the expression level of GPI gene is increased. At the same time, the expression level of other genes of pentose-phosphate cycle enzymes did not change significantly at this experimental condition. It was also shown that upon glutamine deprivation condition the expression level of G6PD and GPI genes is increased and decreased, correspondingly, but other pentose-phosphate cycle genes does not changed significantly in glioma cells. Moreover, the changes in the expression level of these genes upon glutamine deprivation is clearly dependent upon ERN1 signaling enzyme function. Thus, these results demonstrate that suppression of ERN1 signaling enzyme function in glioma cells affects the expression level of three studied genes only and that glutamine deprivation condition induces variable changes in the expression of most investigated genes but preferentially in glioma cells with ERN1 knockdown.
Authors and Affiliations
I. A. GARMASH, D. O. MINCHENKO, A. O. KULINICH, O. V. KOVALEVSKA, О. H. MINCHENKO
Keywords
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