ACUTE TOXICITY OF DIFFERENT SIZES OF SILVER NANOPARTICLES INTRAPERITONALLY INJECTED IN BALB/C MICE USING TWO TOXICOLOGICAL METHODS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 2
Abstract
Objective: This study aimed to evaluate the acute toxicity of intraperitoneally administrated silver nanoparticles (AgNPs) with different particle sizes in BALB/c mice.Methods: Citrate-capped AgNPs were prepared by citrate reduction method and isolated into small particles (average size 20 nm) and large particles (average size 50 nm). The median lethal dose (LD50) of 20 nm and 50 nm AgNPs was estimated using two toxicological methods, classical Dixon's up-and-down method and AOT425statPgm method for up-and-down procedure.Results: The LD50 was evaluated at the dosage level of 169 and 213.8 mg/kg, respectively for 20 nm AgNPs and at the dosage level of 354 and 391.5 mg/kg, respectively for 50 nm AgNPs. The results showed that LD50 obtained by the AOT425statPgm method was in accord with that of the Dixon's method and no significant differences between them (P = 0.06). The size 20 nm AgNPs were more toxic than the size 50 nm AgNPs. The behavioural responses and deviations were dose dependent, increasing by increasing the dose. The anatomical examinations showed that AgNPs were mainly accumulated in liver and spleen of dosed mice.Conclusion: The results suggested that the AOT425statPgm method was an efficient tool and a good alternative method for use in future acute toxicity studies.Â
Authors and Affiliations
Elham A. Elkhawass, Mahmoud E. Mohallal, Maha F. m. Soliman
CYTOTOXICITY EVALUATION OF TITANIUM AND ZINC OXIDE NANOPARTICLES ON HUMAN CELL LINES
Objective: In vitro cytotoxicity evaluation of titanium dioxide, 20 nm (TNP 20) and zinc oxide, 20 nm (ZNP 20) nanoparticles (NP) were tested on different types of human skin (HaCat), lung (A549), liver (Hep G2) and colo...
NEUROTOX: A UNIQUE DATABASE FOR ANIMAL NEUROTOXINS
Objective: A wide range of toxic effects against other species are affected through potential protein molecules called neurotoxins. Among various kinds of toxins, neurotoxins play a critical role in life of other organis...
ANTIOXIDANT ACTIVITIES AND CYTOTOXICITY OF THAI PIGMENTED RICE
Objective: This research quantified the phytochemical profile, antioxidant activities and cytotoxicity of Thai pigmented rice.Methods: Acidified ethanol extracts from seeds of twelve Thai pigmented rice cultivars (six bl...
DESIGN AND EVALUATION OF FAST DISSOLVING ORO-DISPERSIBLE FILMS OF METOCLOPRAMIDE HYDROCHLORIDE USING 32 MULTIFACTORIAL DESIGNS
Objective: The objective of the present work was to develop and optimize fast dissolving orodispersible films containing metoclopramide hydrochloride using 32 multifactorial designs.Methods: The films were prepared by so...
EVALUATION OF IN-VITRO ANTIHELMINTIC PROPERTIES OF THE EXTRACT OF RUSSELIA EQUISETIFORMIS (SCHLECHT. AND CHAM.) SCROPHULARIACEAE
Objective: The present study was aimed to evaluate the in vitro anthelmintic activity of Russelia equisetiformis leaves in comparison with anthelmintic drug Piperazine citrate.Methods: In vitro Anthelmintic activity of d...