Adaptation of the bovine diarrhea virus strain «bug-04» to non-permission animal cell cultures
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2017, Vol 31, Issue
Abstract
Bovine viral diarrhea (mucosal disease, infectious enteritis of cattle) is a common contagious disease affecting different age groups of animals, has a wide range of clinical symptoms, can lead to both immunosuppression and reproductive, respiratory and digestive systems.Laboratory diagnostics of the disease include various methods, among which the virus isolation in the cells culture remains the "gold standard" of the diagnosis until now.The main goal when working with viral material is to preserve its biological properties. The best way to store the virus is lyophilization, but the renewal of the biological properties of the virus from the lyophilic state is always associated with certain difficulties in the accumulation of biomass with high infectious titer. In the work, there may be a need to replace the cell culture, which may cause decreasing of the virus titer.The goals of the work was the investigate the ability of bovine diarrhea virus BUG-04 strain of cultivation in different biological systems.Materials and methods. Cell lines were cultivated in a DMEM nutrient media with 10% fetal bovine serum. Monolayer cell cultivated in plastic culture wells with a volume of 50 cm3 and in 96-well culture plates. Seeding dose was 200000 cells per well. Monolayer cells BGM (African green monkey kidney cells), KST (bovine coronary vessels cells), РК-15 (pig's kidney cells) and FLK (sheep embryonic kidney cells) were infected by strain «BUG-04» in a dose of 0.02-TCID50.Results of research and discussion. It was found that replication induces cytopathic changes in monolayer cultures of transfilled cell lines. The rate of replication and crop of the virus depended on the culture. The first signs of CPE in the infected culture BGM appeared after 72 hours in the first passage, in culture KST on 3rd days on 2nd passage, in culture PK-15, and FLK on 4th days on 3rd passage.Сonclusions and prospects for further research. Based on the results obtained, the BGM cell culture on the 6 passage gives a higher yields of the virus compared to the cell cultures KST, PK-15 and FLK. The obtained data allow expanding the range of studies with bovine diarrhea virus. It is planned to continue the scientific work with the use of other cell culture lines.
Authors and Affiliations
Natalia Gavrasieva, Zinaida Klestova, A Kuzmich
Keywords
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- EP ID EP265897
- DOI 10.31073/vet_biotech31-02
- Views 171
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