Alternate and non-staining methods for confirming cell wall of Gram indeterminate bacteria
Journal Title: Perspectives In Medical Research - Year 2015, Vol 3, Issue 2
Abstract
Clinical microbiologists during their laboratory practice are required to isolate and identify the bacteria from various clinical samples. In most instances gram’s stain is sufficient to know if a bacterium possesses a gram positive cell wall or a gram negative cell wall. Some bacteria produce rough colonies which are hard to emulsify (eg: Actinomyces spp, Corynebacterium spp, propionibacterium spp) and do not reveal the exact gram’s reaction. Few other bacteria are inherently gram variable and pose difficulty in identifying their gram’s reaction. Bacillus spp, Butyrivibrio, and Clostridium spp are gram positive bacteria which show gram negative forms misleading their identification by microbiologists.
Authors and Affiliations
Venkataramana Kandi
Keywords
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