An histochemical study of the effects of immunosuppressive drug azathioprine on the cytoarchitecture of the testes of adult wistar rats (rattus norvegicus)
Journal Title: Scientific Journal of Biological Sciences - Year 2014, Vol 3, Issue 1
Abstract
In this study weemployed some histochemical techniques to evaluate the effect of ascorbic acidon azathioprine-induced alterations in the testis of adult Wistar rats. Maleadult Wistar rats (n=30) with mean weight 210±2.65 g were assigned into fourtreatment (n=24) and control (n=6) groups. Animals were treated with 25 and 50mg/kg azathioprine (AZA) alone (Grps II and IV) and in combination with 25 and50 mg/kg ascorbic acid (Grps III and V) for 21 days while control animalsreceived 1 ml distilled water. 24 h Post-treatment, animals were sacrificed,their testes were excised, weighed and fixed in Bouins’ fluid for histochemicalstudies of reticulin and collagen. The histochemical findings of testissections indicated cyto-architectural distortions in the treated groups whencompared to the control group. This findings show that ascorbic acid at dosesadministered did not ameliorate the damages caused by azathioprine.
Authors and Affiliations
I. O. Onanuga*| Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin; P.M.B. 1515, Ilorin, Nigeria., R. B. Ibrahim| Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin; P.M.B. 1515, Ilorin, Nigeria., R. Jaji-Sulaimon| Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin; P.M.B. 1515, Ilorin, Nigeria., C. N. B. Tagoe| Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin; P.M.B. 1515, Ilorin, Nigeria., O. B. Akinola| Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin; P.M.B. 1515, Ilorin, Nigeria.
Keywords
Related Articles
- EP ID EP1100
- DOI 10.14196/sjbs.v3i1.1168
- Views 461
- Downloads 21
Survey for incidence and severity of bacterial blight of cotton caused by Xanthomonas axonopodis pv. malvacearum in different districts of Marathwada region
An extensive roving survey was conducted in different districts of Marathwada region of Maharashtra State to isolate the pathogen associated with the bacterial blight disease of cotton. In all 76 cotton fields were surve...
Haematologic and Biochemical Indices of Plasmodium falciparum Infected Inhabitants of Owerri, Imo State, Nigeria
The present study seeks to investigate changesin haematologic and biochemical indices of moderately P. falciparum infectedmale inhabitants of Owerri Municipality. Haematologic and biochemical indiceswere estimated by spe...
Microsatellite DNA marker in aquatic organisms
For the last 50 years, attempts have been made to discriminate amongpopulations by using molecular markers. Although some techniques have provedsuccessful in certain circumstances, the consistent trend to newer markersam...
Histological study of the effects of oral administration of datura metel on the visual system of male wistar rats
This study was carried out in order to elucidate some of the effects of oral administration of Cannabis sativa on the visual system of male Wistar rats as marker of toxicity using neurohistochemical study. 12 adult male...
Role of garlic in dyslipidemia: an evidence based review
Rapidly growing morbidity and mortality due to heart disease is a great concerned for scientist. Among the etiology of atherosclerosis, dyslipidemia is a major risk factor. Garlic has active ingredients of allium sativum...