Analyses of Plasmids Harbouring Quinolone Resistance Determinants in Enterobacteriaceae Members
Journal Title: Polish Journal of Microbiology - Year 2017, Vol 66, Issue 4
Abstract
The aim of this study was to explore the plasmid characteristics of eight clinical Enterobacteriaceae strains containing extended broad spectrum beta-lactamases and plasmid-mediated quinolone resistance. Plasmids were transferred by conjugation or transformation and resistance determinants were investigated by PCR. We showed that at least one plasmid harbouring qnrB or qnrS determinant was transferred by conjugation in five isolates. QepA determinant was confirmed to be on a non-conjugative plasmid. We found at least one beta-lactamase gene in seven of the eight clinical isolates having plasmid-mediated quinolone resistance, which indicated that these two resistance determinants were mostly on the same conjugative plasmids.
Authors and Affiliations
Bayri Erac, Fethiye Ferda Yilmaz, Ismail Ozturk, Sabire Sohret Aydemir, Mine Hosgor-Limoncu
Keywords
Related Articles
- EP ID EP245135
- DOI 10.5604/01.3001.0010.7084
- Views 99
- Downloads 0
Morphological and Molecular Characterization of Phoma complanata, a New Causal Agent of Archangelica officinalis Hoffm. in Poland
The paper concerns the fungus Phoma complanata, isolated for the first time in Poland, from the roots and umbels of angelica (Archangelica officinalis) in 2009. The morphology of fungal isolates was tested on standard cu...
Interaction of Gram-Positive and Gram-Negative Bacteria with Ceramic Nanomaterials Obtained by Combustion Synthesis – Adsorption and Cytotoxicity Studies
This paper presents the interactions of Gram-positive ([i]Staphylococcus aureus[/i]) and Gram-negative ([i]Pseudomonas putida[/i]) bacteria with ceramic materials obtained by combustion synthesis. These studies were cond...
<strong>Characterization and Optimization of Biosynthesis of Bioactive Secondary Metabolites Produced by <em>Streptomyces</em> sp. 8812</strong>
The nutritional requirements and environmental conditions for a submerged culture of [i]Streptomyces[/i] sp. 8812 were determined. Batch and fed-batch [i]Streptomyces [/i]sp. 8812 fermentations were conducted to obtain h...
Betaine Improves Polymer-Grade D-Lactic Acid Production by Sporolactobacillus inulinus Using Ammonia as Green Neutralizer
The traditional CaCO3-based fermentation process generates huge amount of insoluble waste. To solve this problem, we have developed an efficient and green D-lactic acid fermentation process by using ammonia as neutralize...
Microbial Biomass and Enzymatic Activity of the Surface Microlayer and Subsurface Water in Two Dystrophic Lakes
Nutrient and organic matter concentration, microbial biomass and activities were studied at the surface microlayers (SML) and subsurface waters (SSW) in two small forest lakes of different water colour. The SML in polyhu...