ANALYSIS OF GROWTH AND SPORULATION OF FILAMENTOUS FUNGI IN DIFFERENT LIQUID MEDIA
Journal Title: International Journal of Medicine and Pharmaceutical Sciences (IJMPS) - Year 2017, Vol 7, Issue 3
Abstract
Composition of culture medium plays a significant role in growth and sporulation of experimental microorganisms. Preparation of most suitable culture media is one of the prerequisites to investigate them. The aim of the present investigation was, to explore four different culture broth namely modified Sabouraud's medium, Richard's medium, Yeast extract medium and Czapek's medium for maximum growth and sporulation of selected keratinophilic (Chrysosporium indicum and Trichophyton terrestre) and dermatophytic fungi (T. rubrum and T. simii), collected from Jaipur district. Initial pH of all media was maintained at 7.5. During present study, the best sporulation was achieved in SDM, followed by RM in all the test fungi. T. simii and T. rubrum showed maximum growth in SDM broth, while in case of T. terrestre and C. indicum, better growth were achieved in Richard's medium. CzM was found to be less suitable for the growth and sporulation of almost all the selected fungi. Final pH of all mediums was found to be changed from initial pH. The information generated will facilitate Mycological research on the fungus causing superficial infection of human being.
Authors and Affiliations
NEETU JAIN, MEENAKSHI SHARMA
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