Analysis of Multi Drug Resistant Mycobacterium tuberculosis Clinical Isolates for Susceptibility to Linezolid and Verapamil using MGIT 960
Journal Title: Saudi Journal of Medical and Pharmaceutical Sciences - Year 2018, Vol 4, Issue 10
Abstract
Pakistan stands among the top five highest tuberculosis burden countries. Emergence of multi drug resistant Mycobacterium tuberculosis limited the spectrum of drugs of choice to treat tuberculosis. Among several, the activation of efflux pumps is one of mechanisms that mediate drug resistance in M. tuberculosis. Here, we tested efflux pump inhibitor verapamil and third line tuberculosis drug linezolid for antimicrobial activity against locally isolated multi drug resistant M. tuberculosis isolates using MGIT 960. M. tuberculosis isolates were collected from two tertiary care hospitals based in Lahore. Out of 100 isolates, 56 % (n=56) were found to be multi drug resistant (MDR). Out of MDR-TB, 5.36% (n=3) were additionally resistant to linezolid and 41.07% (n=17) were resistant to amikacin. One moxifloxacin resistant isolate was identified. In linezolid resistant strains, rplC gene was sequenced where rplC T640C mutation was identified in two of three linezolid resistant strains. The minimum inhibitory concentration of verapamil in 84.5% isolates was 256 μg/ml and the growth of remaining 16.5% isolates was inhibited by verapamil at the concentration of 512 μg/ml. Assessment of synergism between verapamil and other drugs including rifampicin, isoniazid, linezolid, amikacin and moxifloxacin revealed that verapamil at the concentration of 256 μg/ml inhibited the growth of MDR-TB isolates in presence of respective drugs. Our findings suggest that linezolid resistance have started emerging in local isolates. Further studies are required at higher level to identify the exact mechanism.
Authors and Affiliations
Adeel Ahmad, Fizza Mushtaq, Sidrah Saleem, Irfan Ahmad
Keywords
Related Articles
- EP ID EP482568
- DOI -
- Views 111
- Downloads 0
Spectrum of β-Thalassemia and Sickle Cell Anemia Diagnosed by Cation Exchange High Performance Liquid Chromatographyin Different Communities of Mumbai District of Western India- A study from tertiary care hospital
Abstract: Cation exchange high performance liquid chromatography (CE-HPLC) is an important tool in rapid diagnosis of a varied spectrum of Hemoglobinopathies. The aim of the study was to detect hemoglobin variants and he...
Antinociceptive Properties of Desmodium adscendens in Mice
Abstract: This study evaluated the analgesic property of Desmodium adscendens (DA) in mice using the Hot plate test and the Formalin test. Eighteen (18) mice of 15-25g body weight were used for this study. The mice were...
A 27- Year Retrospective Study of Cutaneous Adverse Drug Reaction on In-Patients at Tongji Hospital
Abstract:This study was carried out to investigate the prevalence profile of cutaneous adverse drug reactions (CADRs) on in patients at Tongji hospital. A retrospective study was carried out on in-patients at Tongji medi...
Synthesis and Characterization of Modified Cellulose and Their Use for Removal of Heavy Metal Ions from Aqueous Solution
Abstract:In present study an attempt has been made to prepare new polymers depending on Cellulose viscous pulps to study the adsorption activity for removal of heavy metal ions from single aqueous solution. Barley and Co...
Case-based Learning in Respiratory System for First-year MBBS Students
Abstract:This complete-enumeration, before-and-after type of study (without controls), was conducted in April 2018 on 53 First-year MBBS students (24 females: 45.28% and 29 males: 54.72%) at a medical college in Kalwa, T...