Analysis of Oxidative Stress Markers in Plasma Derived from Individuals Exposed to Ionizing Radiation

Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 15, Issue 5

Abstract

Ionizing radiation can change the redox balance that is disrupted by a decrease in antioxidant activity and an increase in the amount of generated reactive oxygen species, resulting in the oxidative damage at a molecular level and the induction of oxidative stress. Reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) act as indices for the measurement and evaluation of the antioxidant activity and the degree of oxidation in organisms. These tests have the advantage of being rapid, simple and highly reproducible. It is important to determine the oxidative conditions of individuals exposed to ionizing radiation. In the present study, oxidative stress in irradiated mice was assessed using the d-ROMs and BAP tests. The mice were exposed to 0.5, 2 or 7 Gy X-irradiation, and oxidative stress was subsequently assessed at 6, 12, 24 and 72h post-irradiation. The d-ROMs values, which indicate the formation of free radical metabolites, were significantly increased at 24 h post-irradiation with 7 Gy; however, no significant differences were observed between either of the other irradiation groups and the non-irradiated control group at any time-points. The BAP values, which indicate the level of antioxidant activity, were at a minimum at 12h post-irradiation in the groups irradiated with 0.5 and 2 Gy, and subsequently returned to their initial levels. No significant differences were observed between any of the irradiated groups and the control. These results suggest that d-ROMs measurement could serve, to a certain extent, as a prognostic indicator of radiation exposure.

Authors and Affiliations

Ayaka Nishiyama, Masaru Yamaguchi, Takakiyo Tsujiguchi, Ikuo Kashiwakura

Keywords

Related Articles

Essential Quality Analysis Criteria in Forensic Genetics Identification: A Position Statement of Italian Society of Human Genetics

The entry into force of DPR n. 87 of 7th April 2016 relating to the DNA Data Bank requires an implementation upgrade of the Forensic Genetics analysis to ensure the quality of results by the control of the pre-analytical...

Astaxanthin Pattern in Mangroves: A Case of Species- Specificity

Astaxanthin, a naturally occurring carotenoid pigment possessing strong antioxidant property has been detected to play a vital role in the protection against lipid peroxidation and oxidative damage of LDL cholesterol, ce...

Metabolic Surgery for Tackling Type II Diabetes Epidemic

Type II Diabetes is a serious chronic metabolic dysfunction leading to increased morbidity, mortality and cost. Globally an estimated 422 million adults were living with diabetes in 2014, compared to 108 million in 1980....

The Mirizzi Syndrome –Major Cause for Biliary Duct Injury during Laparoscopic Cholecystectomy

After the introduction of laparoscopy as a method of choice for cholecystectomy, the amount of the biliary injury of the main bile duct has raised significantly. Primary cause for this is complicated cholecystitis with g...

A Coinheritance of X-Linked and Autosomal Dominant forms of the Ichthyosis

According to a modern classification, there are two forms of inherited ichthyosises: syndromic and nonsyndromic, each of them consists of more than ten different nosologies [1]. More common types of the ichthyosis are X-...

Download PDF file
  • EP ID EP587601
  • DOI 10.26717/BJSTR.2019.15.002768
  • Views 193
  • Downloads 0

How To Cite

Ayaka Nishiyama, Masaru Yamaguchi, Takakiyo Tsujiguchi, Ikuo Kashiwakura (2019). Analysis of Oxidative Stress Markers in Plasma Derived from Individuals Exposed to Ionizing Radiation. Biomedical Journal of Scientific & Technical Research (BJSTR), 15(5), 11642-11645. https://www.europub.co.uk/articles/-A-587601