Analysis of Oxidative Stress Markers in Plasma Derived from Individuals Exposed to Ionizing Radiation
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 15, Issue 5
Abstract
Ionizing radiation can change the redox balance that is disrupted by a decrease in antioxidant activity and an increase in the amount of generated reactive oxygen species, resulting in the oxidative damage at a molecular level and the induction of oxidative stress. Reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) act as indices for the measurement and evaluation of the antioxidant activity and the degree of oxidation in organisms. These tests have the advantage of being rapid, simple and highly reproducible. It is important to determine the oxidative conditions of individuals exposed to ionizing radiation. In the present study, oxidative stress in irradiated mice was assessed using the d-ROMs and BAP tests. The mice were exposed to 0.5, 2 or 7 Gy X-irradiation, and oxidative stress was subsequently assessed at 6, 12, 24 and 72h post-irradiation. The d-ROMs values, which indicate the formation of free radical metabolites, were significantly increased at 24 h post-irradiation with 7 Gy; however, no significant differences were observed between either of the other irradiation groups and the non-irradiated control group at any time-points. The BAP values, which indicate the level of antioxidant activity, were at a minimum at 12h post-irradiation in the groups irradiated with 0.5 and 2 Gy, and subsequently returned to their initial levels. No significant differences were observed between any of the irradiated groups and the control. These results suggest that d-ROMs measurement could serve, to a certain extent, as a prognostic indicator of radiation exposure.
Authors and Affiliations
Ayaka Nishiyama, Masaru Yamaguchi, Takakiyo Tsujiguchi, Ikuo Kashiwakura
Keywords
Related Articles
- EP ID EP587601
- DOI 10.26717/BJSTR.2019.15.002768
- Views 194
- Downloads 0
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