Analysis of Transforming Growth Factor-Beta1 Gene Polymorphisms in Macedonian Patients with Chronic Periodontitis
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2009, Vol 2, Issue 1
Abstract
Background. The existing conflicting data in the literature about the significance of TGF-beta1 polymorphisms in periodontitis. Aim. To determine whether TGF-beta1 polymorphisms are associated with periodontitis in Macedonian population. Material and methods. The sample consisted of 301 healthy unrelated individuals, and 132 patients with periodontitis. All individuals were of Macedonian origin and nationality and residents of different regions of the Republic of Macedonia. DNA was isolated from peripheral blood leukocytes by the phenol-chlorophorm extraction method. Cytokine polymorphism genotyping was performed by PCR-SSP (Heidelberg kit). The population genetics analysis package (PyPop) was used for analysis of the data. Crude odds ratios, were calculated with 95% confidence interval. P<0.05 was considered statistically significant. Results. Significant negative (protective) association between the Macedonian patients with periodontitis and: 1) cytokine genotypes TGF-beta1 cdn 10/C:T and TGF-beta1 cdn 25/G:G; 2) cytokine haplotypes TGF-beta1/TG and cytokine haplotype zygotes TGF-beta1/CG:TG were found. Positive (susceptible) association was found with: 1) cytokine alleles TGF-beta1 cdn 10/C and TGF-beta1 cdn 25/C; 2) cytokine genotypes TGF-beta1 cdn 10/C:C and TGF-beta1 cdn 25/C:G; and 3) cytokine haplotypes TGF-beta1/CC and cytokine haplotype zygotes TGF-beta1/ CC:CG. Conclusion. It is concluded that polymorphisms of TGF-beta1 gene are associated with an increased risk of chronic periodontitis in Macedonian population.
Authors and Affiliations
Aneta Atanasovska-Stojanovska| Dental Clinical Center, Department of Oral Pathology and Periodontology, Faculty of Stomatology, University “Ss. Kiril and Metodij”, Skopje, Republic of Macedonia and , Dejan Trajkov| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Kiril and Metodij”, Skopje, Republic of Macedonia, Mirjana Popovska| Dental Clinical Center, Department of Oral Pathology and Periodontology, Faculty of Stomatology, University “Ss. Kiril and Metodij”, Skopje, Republic of Macedonia and , Mirko Spiroski| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Kiril and Metodij”, Skopje, Republic of Macedonia
Keywords
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- EP ID EP8656
- DOI 10.3889/MJMS.1857-5773.2009.0029
- Views 375
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