ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF TRABECTEDIN IN BULK AND PARENTERAL DOSAGE FORM BY RP-HPLC
Journal Title: Indo American Journal of Pharmaceutical Sciences - Year 2018, Vol 5, Issue 3
Abstract
A new RP-HPLC method for the quantitative determination of Trabectedin was developed and validated as per ICH guidelines. The drugs were injected into Zorbax SB, C18,(150x4.6mm); 3.5µm column maintained at ambient temperature and effluent monitored at 215nm. The mobile phase consisted of phosphate buffer (pH 3.0) and Acetonitrile in the ratio of 70:30 V/V. The flow rate was maintained at 0.8 ml/min. The calibration curve for Trabectedin was linear from 50-175µg/ml (r 2 for Trabectedin = 1 ). The proposed method was adequate, sensitive, reproducible, accurate and precise for the determination of Trabectedin in bulk and pharmaceutical dosage forms. Keywords: Trabectedin, Linearity , Accuracy, Validation.
Authors and Affiliations
Keywords
Related Articles
- EP ID EP273977
- DOI -
- Views 324
- Downloads 0
STUDIES ON QUALITATIVE PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF Piper nigrum
The Piper nigrum L. plant materials such as [leaf and seed] were collected and allowed to shade dry to remove moisture content. The dried samples were used for further studies. The powdered plant materials were filled se...
PRELIMINARY STUDIES OF PHYTOCHEMICAL INVESTIGATION ON COASTAL MEDICINAL PLANTS OF BOLOOR, MANGALORE
The present study was aimed to screen the phytochemicals present in five different solvent extracts of Ipomoea –per caprea, Premna serratifolia and Synadenium grantii.Qualitative estimation of three coastal medicinal pla...
FORMULATION AND EVALUATION OF CHITOSAN NANOPARTICLE LOADED WITH PHYLLANTHIN
Objective: The aim of the work was to formulate and evaluate chitosan nanoparticle loaded with Phyllanthin. Methods: Chitosan nanoparticle loaded with phyllanthin were prepared by ionic chelation method using chitosan so...
CURRENT TREND IN PRESCRIBING PATTERN OF ANTIHYPERTENSIVE DRUGS IN A TERITIORY CARE TEACHING HOSPITAL: A PROSPECTIVE OBSERVATIONAL STUDY
Background: Hypertension is one of the major chronic diseases resulting in high mortality and morbidity worldwide. A total of 972 million people were affecting worldwide. Prevalence of Hypertension in India is reported t...
DESCRIPTIVE STUDY KNOWING THE PATIENTS LOAD IN THE NEONATAL ICU AT THE TERTIARY CARE HOSPITAL LAHORE
Objective: To Know the Number of patients referred to tertiary hospital neonatal intensive care unit (NICU) in Lahore, disease model and outcome determination. Study Design: A descriptive study Location and duration: The...