ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF ORNIDAZOLE IN TABLET DOSAGE FORM BY ZERO ORDER UV SPECTROSCOPY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 3
Abstract
Objective: To develop and validate two simple and cost effective spectrophotometric methods for the determination of Ornidazole (OND) in pure form and in pharmaceutical tablet dosage form.Methods: Single point standardization method was used for quantitative estimation of OND. In method 1 absorbance was measured at 311 nm (λmax of OND in methanol) using methanol as a solvent system and in method 2 absorbance was measured at 277 nm (λmax of OND in 0.1 N HCl) using 0.1 N HCl as the solvent system.Results: The two methods obeyed Beer’s law in the concentration range of 3-18 µg/ml (methanol) and 10-35 µg/ml (0.1 N HCl) respectively. The methods were validated for parameters such as linearity, accuracy, and precision, limit of detection (LOD), limit of quantification (LOQ), robustness and ruggedness as per ICH guidelines.Conclusion: The developed methods were used for determining the content of OND in commercial tablets. The proposed methods were thus found to be suitable for quantitative estimation of OND in bulk and tablet formulation without any interference of the excipients and can be employed for quality control of Ornidazole in tablet dosage form.Keywords: Ornidazole, Validation, Single point standardization, ICH guidelines
Authors and Affiliations
Jyotsna Patil, Malathi Raghunath, Shweta Patil
Keywords
Related Articles
- EP ID EP577800
- DOI -
- Views 68
- Downloads 0
MEDICINES WASTE POLICIES AND THE POPULATION KNOWLEDGE IN BRAZIL
Objective: To investigate the degree of knowledge of the patients enrolled in the Unified Health System of the Medicine School of ABC (Faculdade de Medicina do ABC) regarding the proper use and disposal of medicaments....
IN VIVO TOXICITY STUDIES OF BIOSYNTHESIZED SILVER NANOPARTICLES USING BRASSICA OLERACEAE IN ZEBRA FISH MODEL
Objective: Nanotechnology opens new applications in many fields including medicine, material science and various technologies. The aim of the current study is to synthesise nanoparticles of Brassica oleraceae by green ch...
COST OF THERAPY INCURRED FOR TUBERCULOSIS PATIENTS RECEIVING DIRECTLY OBSERVED THERAPY (DOT)
Objective: To estimate the cost of therapy of tuberculosis patients registered under DOTS program in the treatment of tuberculosis.Methods: A prospective observational study was conducted over a period of nine months in...
IMPROVEMENT OF GLICLAZIDE’S DISSOLUTION RATE BY USING SURFACE SOLID DISPERSION WITH AVICEL PH 101
Objective: The aim of this study was to improve the dissolution rate of gliclazide by developing a surface solid dispersion (SSD) with Avicel PH 101 as a carrier.Methods: Solid dispersions of gliclazide were prepared, wi...
QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP ANALYSIS OF NOVEL PYRAZOLINE DERIVATIVES USING K NEAREST NEIGHBOUR MOLECULAR FIELD ANALYSIS METHOD
Objective: Malonyl CoA decarboxylase (MCD) enzyme plays important role in fatty acid and glucose oxidation. Inhibition of MCD might turn to a novel approach to treat ischemia. The main objective of this research article...