Analytical Method Development and Validation of Secnidazole Tablets by RP-HPLC
Journal Title: Journal of Pharmaceutical Sciences and Research - Year 2010, Vol 2, Issue 7
Abstract
A simple, rapid, sensitive specific, accurate, RP-HPLC method was developed and validated for the determination of secnidazole in formulation. The method utilized HPLC (Alliance 2695 with 2487PDA) model and a column (Inertsil, ODS- 3V, 250 4.6mm, 5). The mobile phases were comprised of buffer: 0.01M KH2PO4 : ACN (85:15). Validation experiments were performed to demonstrate system suitability, specificity, precision, linearity and range, accuracy, ruggedness and robustness. The developed method was linear over the concentration range of 30-70g/ml-1. The method showed good recoveries (98.0 – 102.0%) and the relative standard deviations of intra and inter-day . assay were 0.008 and 0.12% respectively. The proposed method is precise, accurate, selective and rapid for the determination of secnidazole for the quality control test
Authors and Affiliations
Nasiruddin Ahmad Farooqui , A. Anton Smith , H. K. Sharma , R. Manavalan
Keywords
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