ANAYTICAL METHOD DEVEOPMENT AND VAIDATION OF BUTYLATED HYDROXYTOLUENE IN ROSUVASTATIN TABLETS BY USING RP-HPLC
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 5
Abstract
An RP-HPLC method was developed and validated for the estimation of Butylated Hydroxy toluene in Rosuvastatin Tablets. The chromatographic system was equipped with Hypersil BDS, C18, 250 X 4.6 mm, internal diameter with 5 micron particle size column and PDA detector set at 277nm, in conjunction with a mobile phase of Water and Methanol in the ratio of 100:900 at a flow rate of 0.8 ml/min. The retention time of Butylated Hydroxy toluene was found to be 7 minute. The separation was performed at ambient temperature. Linearity was observed in the concentration range of 2.5-7.5μg/ml with correlation co-efficient 0.999.Percentage recovery obtained 100.3%-100.8%. The percentage assay was found to be 99.8% .The proposed method is precise, accurate, selective and rapid for the determination of Butylated Hydroxytoluene in Rosuvastatin Tablets. The proposed method is optimized and validated as per the International Conference on Hormonization (ICH) Guidelines.
Authors and Affiliations
Alagar Raja| Department of pharmaceutical analysis & Quality assurance, Nalanda College of pharmacy Cherlapally, Nalgonda-508001, Telangana, South India., M, Bhavani. R| Department of pharmaceutical analysis & Quality assurance, Nalanda College of pharmacy Cherlapally, Nalgonda-508001, Telangana, South India., David Banji| Department of pharmaceutical analysis & Quality assurance, Nalanda College of pharmacy Cherlapally, Nalgonda-508001, Telangana, South India., K. N. V. Rao| Department of pharmaceutical analysis & Quality assurance, Nalanda College of pharmacy Cherlapally, Nalgonda-508001, Telangana, South India., Selva kumar. D| School of Pharmacy, Taylors University, Subangjaya, Malaysia.
Keywords
Related Articles
- EP ID EP3429
- DOI -
- Views 397
- Downloads 24
ARTERIAL VARIATIONS OF HUMAN SUPRARENAL GLAND- A CASE REPORT
Knowledge of anomalous arteries to the suprarenal gland (SRG) is significant for surgeons and intervention radiologists. These variations cannot be ignored. During routine dissection in male cadaver, following variation...
Pharmacognostical, Phytochemical, Pharmacological and Toxicological Review on Trichosanthesanguina Linn
Traditional medicine is widely spread globally and it is the almost exclusive source of primary health care for 80% of the world’s population. Herbal medicines are regarded by the public and some health care providers to...
COMPARISON OF THE THERAPEUTIC ACTIONS OF TAURINE, METHYLSULFONYLMETHANE AND SILYMARIN AGAINST ACETAMINOPHEN-INDUCED NEURO- AND HEPATO-TOXICITY IN ADULT MALE ALBINO RATS.
To evaluate the neuro- and hepato-curative effects of taurine (TAU) and methylsulfonylmethane (MSM) in acetaminophen- induced neuro- and hepato- toxicity in adult male albino rats. Silymarin (SLN) was used as reference d...
ATHEROSCLEROSIS OF AORTA - AN AUTOPSY STUDY
Background and Objectives: Atherosclerosis is a complex and common disease contributing to increased mortality and morbidity. The exact global incidence of atherosclerosis is beyond calculation. Autopsy studies can prov...
ELECTROCHEMICAL PEROXIDASE SENSOR BASED ON MAGNESIUM OXIDE-CHITOSAN NANOBIOCOMPOSITE FILM
Nanoparticles play a major role in development of new hybrid composites for various engineering applications. A simple and novel amperometric biosensor for hydrogen peroxide detection is proposed. It is based on the immo...