Anti-epileptic drugs inhibit viability of synoviocytes in vitro
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2013, Vol 20, Issue 3
Abstract
Introduction and objective: The hyperplasia of synovial fibroblasts is considered to be essential for the evolution of joint destruction in rheumatoid arthritis (RA). Previously, we reported that anti-rheumatic drugs, both COX inhibitors and disease-modifying anti-rheumatic drugs inhibit proliferation of synoviocytes[i] in vitro[/i]. The presented study investigates the effect of anti-epileptic drugs on the viability and proliferation of synovial fibroblasts [i]in vitro[/i]. Methods: Experiments were conducted on human synoviocytes derived from an RA patient and rabbit synoviocytes cell line HIG-82. Cell proliferation and viability were assessed by means of BrdU assay and MTT assay, respectively. The IC[sub]50[/sub] value (the concentration of drug necessary to induce 50% inhibition) together with confidence limits was calculated. Results: Carbamazepine inhibited proliferation of human fibroblasts and viability of HIG-82 with IC 50 values of 86 µM and 82 µM, respectively. Diphenylhydantoin, valproate and phenobarbital inhibited viability of HIG-82 cells with the IC[sub]50[/sub] values of 110, 500 and 1031 µM, respectively. Conclusion: Based on these findings, it can be suggested that anti-epileptic drugs may have a disease-modifying effect on rheumatoid synovial proliferation.
Authors and Affiliations
Jolanta Parada-Turska, Patrycja Nowicka-Stążka, Maria Majdan, Mirosław Jabłoński, Waldemar A. Turski, Wojciech Rzeski
Keywords
Related Articles
- EP ID EP80667
- DOI -
- Views 140
- Downloads 0
Westernization of dietary patterns among young Japanese and Polish females – a comparison study
Introduction and objective. Nowadays, the process of the westernization of eating habits is perceived to be one of the main causes of epidemics of civilization diseases, such as metabolic syndrome. The aim of the study w...
In vivo investigations of neurotensin receptors in adipocytes, hepatocytes and enterocytes of rat
Introduction: Atherosclerotic vascular disease is currently the biggest threat and the highest cause of death worldwide, approaching almost 60%.The development of atherosclerosis is affected by ecological factors associa...
Hyperprolactinemia diagnostics – dilemmas over optimal selection of prolactinemia time points
Introduction. Laboratory discrimination of pathologic hyperprolactinemia is an important step in the diagnosis of pathology influencing overall health and fertility. A major issue is the choice of time and circumstances...
<em>Brucella suis </em>biovar 2 isolations from cattle in Poland
Bovine brucellosis is an infectious disease caused by bacteria of the [i]Brucella genus[/i], primarily by [i]B. abortus[/i], less frequently by [i]B. melitensis[/i], and occasionally by [i]B. suis[/i]. In the European Un...
Causes and consequences of head injuries among rural inhabitants hospitalised in a Multi-organ Injury Ward. II. Circumstances, types and consequences of head injuries.
The scope of problems concerning head injuries was investigated among rural patients, compared to urban population, from the aspect of their incidence in both populations, as well as an attempt to perform a multi-variabl...