Anti-inflammatory Effects and Acute Toxicity of Methanol Stem Bark Extract of Morus mesozygia Stapf.
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 13, Issue 6
Abstract
Aim: Morus mesozygia is a specie of the Morus family in Africa that is traditionally used for the treatment of rheumatism. Until this moment, there is no available literature on the scientific evidence of the in vivo anti-inflammatory effects. The aim is to determine the safety of this extract after acute exposure and evaluate the anti-inflammatory effects in vitro and in vivo. Methodology: The phytochemical screening of the powdered stem bark was determined following standard procedures. Extraction in methanol yielded a brownish extract, which was subjected to total phenolic and flavonoid contents estimation. Antioxidant activity was determined by DPPH free radical scavenging assay. Acute oral toxicity effects was evaluated in female mice and signs of toxicity was observed for 14 days. Anti-inflammatory activity was evaluated using the carrageenan-induced paw oedema and in vitro membrane stabilizing activity assay in rat erythrocytes. Results: Preliminary phytochemical analysis revealed the presence of flavonoids, saponins, tannins, alkaloids, anthraquonones, and cardiac glycosides. Ratio of total phenolic to total flavonoid content was determined to be 0.53. MEMm showed strong antioxidant activity comparable to ascorbic acid in DPPH free radical scavenging activity assay. No signs of acute toxicity was observed indicating the LD50 is greater than 5000 mg/kg. MEMm (200 and 400 mg/kg) significantly (p < 0.05) reduced oedema formation in rat paw, and showed concentration dependent membrane stabilizing activities in rat erythrocytes. Conclusion: The present study shows that stem bark methanol extract of Morus mesozygia is safe and contains bioactive constituents with antioxidant, anti-inflammatory and membrane stabilizing properties.
Authors and Affiliations
Kudirat O. Oshiomah, Oyinlade A. Odujoko, Abayomi M. Ajayi
Keywords
Related Articles
- EP ID EP342546
- DOI 10.9734/BJPR/2016/28746
- Views 115
- Downloads 0
Cytoprotective and Antioxidant Properties of the Stem Bark Aqueous extract of Khaya grandifoliola (Meliaceae) in Rats
Aims: To evaluate the qualitative chemical composition of the aqueous extract of the stem bark of Khaya grandifoliola and test the antiulcer actions on gastric lesions induced by HCl/Ethanol, HCl/Ethanol/Indomethacin, in...
In vitro Cytotoxic Activity of Hydro Ethanolic Extract of Delonix regia (Bojer ex. Hook.) Flowers on Cancer Cell Lines
Aims: To evaluate the In vitro cytotoxic effects of the hydro ethanolic extract (HEE) of the Delonix regia flowers against different cell lines. Methodology: The dried Delonix regia flowers are subjected to soxhlet extra...
Identification of Phenolic Compounds in Hibiscus sabdariffa Polyphenolic Rich Extract (HPE) by Chromatography Techniques
The study was designed to determine the phenolic constituents of Hibiscus sabdariffa polyphenolic rich extract (HPE), (a group of phenolic compounds occurring in the dried calyx of the red variety of Hibiscus sabdariffa)...
A New Bioanalytical Method Development & Validation for Simultaneous Estimation of Esomeprazole and Naproxen in Human Plasma by Using RP-HPLC
Aim: A new reverse phase high performance liquid chromatography (RP-HPLC) method for the quantitative determination of Esomeprazole and Naproxen in human plasma was developed and validated as per U...
Preliminary Phytochemical Screening and In vitro Antioxidant Activity of Methanolic Extract of Tropaeolum majus L. Seed
Objective: To investigate the preliminary phytochemical screening and in vitro antioxidant activity of methanol extract of Tropaeolum majus L. (T. majus) seeds. Methods: Phytochemical screening was performed by using st...