Antibacterial screening of four local plants using an indicator-based microdilution technique
Journal Title: African Journal of Traditional, Complementary and Alternative Medicines - Year 2005, Vol 2, Issue 3
Abstract
The antibacterial activities of the plants, [i]Mitragyna inermis, Morinda lucida, Khaya senegalensis[/i] and [i]Nauclea latifolia[/i] were investigated using an indicator-based microdilution technique. The extracts of the plants in water, methanol, chloroform and petroleum ether inhibited growth of bacteria in broth cultures. [i]M. inermis[/i] with the lowest minimal inhibitory concentration (MIC) of 0.03g/ml was the bacteriologically most active plant. The chi-square value (10.25) and F-statistic value (4.36) were significant at less than 0.05 level and implied that inhibition of bacterial growth was significantly associated with the type of plant investigated. The R value obtained on regressing bacterial inhibition on the independent variables – type of plant, plant part (leaf or stem bark) and extraction solvent used - was 0.57 (p < 0.01 level), and means that the independent variables put together influenced inhibition of bacterial growth . The findings of this study suggest the effectiveness of the microdilution technique in the phytochemical screening of plants for antibacterial activities.
Authors and Affiliations
E. Umeh, H. O. Oluma, J. Igoli
Keywords
Related Articles
- EP ID EP159750
- DOI -
- Views 115
- Downloads 0
Antitrichomonal activity of 1,3-diaryl-2-propen-1-ones on <i>trichomonas gallinae</i>
1,3-diaryl-2-propen-1-ones were synthesized by the Claisen - Schmidt condensation method. T. gallinae parasites isolated from domestic pigeon were cultured in vitro. The in vitro susceptibility of T. gallinae was evaluat...
ANTICESTODAL EFFICACY OF FOLKLORE MEDICINAL PLANTS OF NAGA TRIBES IN NORTH-EAST INDIA
The anticestodal efficacy of nine plants that are used in the indigenous system of medicine by Naga tribes in north-east India to cure intestinal-helminth parasitic infections was tested employing Raillietina echinobothr...
ZINGIBER OFFICINALE: EVAULATION OF ITS NOOTROPIC EFFECT IN MICE
Dementia is one of the age-related mental problems, and a characteristic symptom of Alzheimer’s disease. Nootropic agents and cholinesterase inhibitors like donepezil® are clinically used in situations where there is org...
Gossypol content on leaves and seeds from some wild <i>malvaceae</i> species
Ten Malvaceae species were analysed for their seed and leaf gossypol content by HPLC. The results showed that gossypol is common in most of the Malvaceae family, but its concentration varies among the species and also am...
TRADITIONAL MEDICINE IN LATE PREGNANCY AND LABOUR: PERCEPTIONS OF KGABA REMEDIES AMONGST THE TSWANA IN SOUTH AFRICA
In South Africa most black women use antenatal care services and deliver in clinics, and a considerable number complement this use of formal health services with traditional medicine. This study reported here examined th...