Anticancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.)
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2017, Vol 10, Issue 6
Abstract
Objective: To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.). Methods: Anticancer activity of fungal extracts was determined by observing its toxicity against MCF-7 (Michigan Cancer Foundation-7) cells in vitro by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. At an extract concentration of 100 mg/mL, 4 isolates out of 12 showed high activity against the cancer cell growth. The four isolates were then selected for further IC50 determination, by measuring the inhibition of cancer cell proliferation at extract concentration of 25 mg/mL, 50 mg/mL, 100 mg/mL, 200 mg/mL and 400 mg/mL. Results: Results showed that isolate Sir-G5 had the highest anticancer activity with an IC50 of 19.20 mg/mL. The best isolates were screened again using a normal cell (Chang cells) to determine its toxicity against normal cells. Results indicated that the extracts do not affect the proliferation of normal cells. Molecular identification showed that the fungal isolate Sir-G5 has a close relationship with Phomopsis sp. Conclusions: The endophytic fungi isolated from soursop leaf has the potential to be used as a source of anticancer agents.
Authors and Affiliations
I Made Artika
Keywords
Related Articles
- EP ID EP267433
- DOI 10.1016/j.apjtm.2017.06.004
- Views 127
- Downloads 0
Rescue effects of aqueous seed extracts of Foeniculum vulgare and Carum carvi against cadmium-induced hepatic, renal and gonadal damage in female albino rats
Objective: To investigate the protective effects of aqueous seed extracts of fennel “Foeniculum vulgare” (FVE) and caraway “Carum carvi” (CCE) on liver, kidney and reproductive organs in female rats against cadmium chlor...
Chemical analysis and in vitro antimicrobial effects and mechanism of action of Trachyspermum copticum essential oil against Escherichia coli
Objective: To find a natural plant essential oil (EO) with excellent antimicrobial effects on food-borne bacteria and to explore the mechanism of its antimicrobial function against Escherichia coli (E. coli). Methods: Th...
Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) polymorphism as a genetic marker of cerebral malaria in Thai population
Objective: To know whether the effect of interferon-induced protein with tetratricopeptide repeats (IFIT) 1 polymorphism influences the susceptibility of cerebral malaria outcome. Methods: Case-control association study...
Chloride channel involved in the regulation of curcumin-induced apoptosis of human breast cancer cells
Objective: To investigate the role of ClC-3 chloride channel in the proliferation of breast cancer cell line Mcf-7 treated with curcumin and its specific mechanism. Methods: MTT assay was used to detect the effect of chl...
Andrographolide inhibits chikungunya virus infection by up-regulating host innate immune pathways
Objective: To investigate the therapeutic efficacy of andrographolide, a plant derived compound, against chikungunya virus (CHIKV) infection. Methods: Using flow cytometry and immunoblotting assay, in vitro viral protein...