Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells

Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2018, Vol 8, Issue 8

Abstract

Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts (aerial, leaf, stem) of whole plant on HT-29 colon cancer cell lines. Three different extracts from the plant parts were prepared by maceration technique using 80% ethanol. The anticancer activities were determined using MTT, clonogenic, cell motility and AOPI assay. The chemical composition profiling was analyzed by GC-MS. Results: Among three plant part extracts, leaf extract greatly suppressed the growth of colon cancer cells in time and dosage-dependent manner, followed by aerial and stem. The cytotoxicity results were rationalized with clonogenic, cell motility and AO/PI assay, where extract showed the most active activity compared to aerial and stem extracts. GC-MS analysis of leaf extract showed there were various recognized anti-cancer, anti-oxidant and anti-inflammatory compounds. Conclusions: Amid the screened extracts, the leaf extract exhibits the credible cytotoxic, anti-proliferative and apoptotic activity and hence, our findings call for additional research to conclude the active compounds and their mechanisms determining the apoptotic activity.

Authors and Affiliations

Arulselvan Palanisamy

Keywords

Related Articles

Fate of polyphenols in pili (Canarium ovatum Engl.) pomace after in vitro simulated digestion

Objective: To evaluate the stability and bioavailability of polyphenols in pili (Canarium ovatum Engl.) pomace during simulated in vitro digestion. Methods: Freeze-dried pili pomace was subjected to in vitro digestion si...

Genetic analysis of maturity and flowering characteristics in maize (Zea mays L.)

Objective To elucidate the pattern of inheritance and determine the relative magnitude of various genetic effects for maturity and flowering attributes in subtropical maize. Methods Four white grain maize inbred lines...

Incidence and histopathology of encysted progenetic metacercaria of Clinostomum complanatum (Digenea: Clinostomidae) in Channa punctatus and its development in experimental host

Objective To study the incidence of encysted progenetic metacercariae of Clinostomum complanatum (C. complanatum) in Channa punctatus (C. punctatus), associated histopathology and the experimental infection to laborator...

Larvicidal activity of Xenorhabdus and Photorhabdus bacteria against Aedes aegypti and Aedes albopictus

Objective: To evaluate the efficacy of symbiotic bacteria, Xenorhabdus indica, Xenorhabdus stockiae, Photorhabdus luminescens subsp. akhurstii and Photorhabdus luminescens subsp. hainanensis as a larvicide against Aedes...

Establishment of an early warning system for cutaneous leishmaniasis in Fars province, Iran

Objective: To establish an early warning system for cutaneous leishmaniasis in Fars province, Iran in 2016. Methods: Time-series data were recorded from 29 201 cutaneous leishmaniasis cases in 25 cities of Fars province...

Download PDF file
  • EP ID EP369572
  • DOI 10.4103/2221-1691.239427
  • Views 70
  • Downloads 0

How To Cite

Arulselvan Palanisamy (2018). Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells. Asian Pacific Journal of Tropical Biomedicine, 8(8), 394-402. https://www.europub.co.uk/articles/-A-369572