Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells

Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2018, Vol 8, Issue 8

Abstract

Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts (aerial, leaf, stem) of whole plant on HT-29 colon cancer cell lines. Three different extracts from the plant parts were prepared by maceration technique using 80% ethanol. The anticancer activities were determined using MTT, clonogenic, cell motility and AOPI assay. The chemical composition profiling was analyzed by GC-MS. Results: Among three plant part extracts, leaf extract greatly suppressed the growth of colon cancer cells in time and dosage-dependent manner, followed by aerial and stem. The cytotoxicity results were rationalized with clonogenic, cell motility and AO/PI assay, where extract showed the most active activity compared to aerial and stem extracts. GC-MS analysis of leaf extract showed there were various recognized anti-cancer, anti-oxidant and anti-inflammatory compounds. Conclusions: Amid the screened extracts, the leaf extract exhibits the credible cytotoxic, anti-proliferative and apoptotic activity and hence, our findings call for additional research to conclude the active compounds and their mechanisms determining the apoptotic activity.

Authors and Affiliations

Arulselvan Palanisamy

Keywords

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  • EP ID EP369572
  • DOI 10.4103/2221-1691.239427
  • Views 73
  • Downloads 0

How To Cite

Arulselvan Palanisamy (2018). Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells. Asian Pacific Journal of Tropical Biomedicine, 8(8), 394-402. https://www.europub.co.uk/articles/-A-369572