Antifertility properties of Centella asiatica ethanolic extract as a contraceptive agent: Preliminary study of sperm proteomic

Journal Title: Asian Pacific Journal of Reproduction - Year 2017, Vol 6, Issue 5

Abstract

Objective: To investigate the antifertility properties of Centella asiatica L. ethanolic extract and identify the sperm proteomic changes in treated male rats. Methods: A total of 32 male Sprague-Dawley rats were divided into two groups: control group (n=16) received distilled water and treatment group (n=16) which received 300 mg/kg of Centella asiatica L. extract for 42 d. After the treatment period, the number of implantation sites was recorded and the sperm proteomic changes were analysed by 2D gel electrophoresis. In addition, the expression of protein spots was quantified by MALDI-TOF analysis. Results: Centella asiatica L. extract resulted in low number of implantation sites in the treatment group (100.00±2.82) compared to the control group (183.00±2.14). The percentage of infertile male rats in the treatment group was higher (43.75%) compared to the control group (18.75%). Proteomic analysis showed the expression of protein spots identified in the treatment group decreased with 234 spots compared to the control group with 282 spots. Conclusions: The results revealed that there was antifertility activity in the male rats with the administration of Centella asiatica L. ethanol extract and the identified proteins could provide understanding on the adverse effect of Centella asiatica in male reproductive system.

Authors and Affiliations

Irfan Yunianto, Nurul Ain Kamar Bashah, Mahanem Mat Noor

Keywords

Related Articles

The methanolic extract of Guibourtia tessmannii (Caesalpiniaceae) improves sexual parameters in high fat diet-induced obese sexually sluggish rats

Objective: To evaluate the effects of the methanolic extract of Guibourtia tessmannii (G. tesmannii) on sperm parameters, lipid profile and testosterone level in obese rats. Methods: A total of 193 male Wistar rats were...

Factors affecting in-vivo fertility of crossbred Egyptian - Italian buffalo semen

Objective: To assess the effect of region, season and year of insemination on in-vivo fertility of Italian-Egyptian crossbred buffalo semen. Methods: A total number of 4 799 female buffaloes were inseminated by frozen se...

Oestrous cycle of Wistar rats altered by sterol and triterpenes rich fraction of Adansonia digitata (Linn) root bark - A scientific rationale for contraceptive use

Objective: To investigate the effect of different fractions from the aqueous methanolic root bark of Adansonia digitata (Linn) on the estrous cycle of female Wistar rats and to elucidate their phytochemical content. Meth...

Genetic polymorphism and natural fertility in women

Objective: To investigate the cooperative interaction among five genetic systems (phosphoglucomutase locus 1, adenosine deaminase locus 1, acid phosphatase locus 1, adenylate kinase locus 1, and haptoglobin) concerning t...

Anti-VDAC3 recombinant antibody decreased human sperm motility and membrane integrity: A potential spermicide for contraception

Objective: To express recombinant protein that comprises an important fragment of human sperm specific voltage dependent anion channel 3 (VDAC3) protein as a potential molecule for generation of antibody, which can affec...

Download PDF file
  • EP ID EP243320
  • DOI 10.4103/2305-0500.215931
  • Views 225
  • Downloads 0

How To Cite

Irfan Yunianto, Nurul Ain Kamar Bashah, Mahanem Mat Noor (2017). Antifertility properties of Centella asiatica ethanolic extract as a contraceptive agent: Preliminary study of sperm proteomic. Asian Pacific Journal of Reproduction, 6(5), 212-216. https://www.europub.co.uk/articles/-A-243320