Antihypertensive efficacy of extract of Hedera helix in high salt-induced hypertensive Sprague-Dawley rats
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2018, Vol 11, Issue 8
Abstract
Objective: To explore the antihypertensive effect of extracts from the leaves of Hedera helix (H. helix) on normotensive and hypertensive rats in-vivo followed by vasodilatory studies in-vitro. Methods: The crude methanolic extract was prepared and the activity directed fractionation was carried out. Spectrophotometric analysis of total phenolic and flavonoid content was also done. HPLC analysis was performed for the detection of hederacoside C. In-vivo blood pressure study was carried out in normotensive and high salt-induced hypertensive Sprague-Dawley rats. Isolated aortic tissues from rat and rabbit were used for in-vitro studies. The effects were recorded and analyzed through PowerLab data acquisition system. Results: Crude extract of H. helix (1-30 mg/kg) decreased blood pressure to greater extent in high salt-induced hypertensive rats in-vivo compared to the normotensive [Max. fall (58.59±0.02) mmHg vs. (67.53±3.07) mmHg]. The n-hexane, chloroform, ethyl acetate and aqueous fractions were also checked. These fractions were more effective in hypertensive rats. Aqueous fraction was more potent and n-hexane the least. In isolated rat aortic rings precontracted with phenylephrine, crude extract induced endothelium-dependent effect. The endothelium-dependent component of vasodilatory effect was ablated with L-NAME, and denudation of endothelium. The aqueous fraction was most potent vasodilator. In aortic rings from hypertensive rats, extract and fractions produced partial endothelium-independent effect which was not affected by pretreatment with L-NAME, indicating endothelium dysfunction in the hypertensive rats and suggesting additional vasodilatory mechanisms. In rabbit aorta, the extract and fractions also inhibited phenylephrine and high K+ -induced precontractions, and shifted Ca++ concentration-response curves. Conclusions: Our findings indicate that extract and fractions of H. helix are antihypertensive remedies, which is the outcome of vasodilatory effect. This vasodilatory effect is mediated through nitric oxide and Ca++ antagonism.
Authors and Affiliations
Abdul Jabbar Shah
Keywords
Related Articles
- EP ID EP376176
- DOI 10.4103/1995-7645.240083
- Views 91
- Downloads 0
Effects of albendazole, artesunate, praziquantel and miltefosine, on Opisthorchis viverrini cercariae and mature metacercariae
Objective: To explore larvicidal effects of anthelmintic drugs on Opisthorchis viverrini (O. viverrini) for alternative approach to interrupting its cycle for developing a field-based control program. Methods: The larvic...
Chikungunya infection: A potential re-emerging global threat
Infectious diseases are indeed a lifelong threat to everyone irrespective of age, sex, lifestyle and socio-economic status. The infectious diseases have persisted among the prominent causes of death globally. Recently, r...
Evaluation of interleukin 8 +2767 A/T polymorphism in visceral leishmaniasis
Objective: To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL pathogenesis among Iranian patients. Methods: Three groups including patients with VL clinical presentation and leish...
Correlation between quality of drinking water sources and meteorological factors in 2013-2017, Haikou, South China
Objective: With the increasing attention to health influence by ecology environment and climate changes, it is important to explore the characters of drinking water quality and meteorological factors at tropical areas of...
Prevalence of hydatidosis in livestocks in Chakwal District of Pakistan
Background: Hydatidosis is a zoonotic parasitic disease and a serious human and animal health problem in many endemic areas of the world. It is caused by the tapeworm parasite of Echinococcus granulosus sensu lato. This...