Antimalarial efficacy of Nyctanthes arbor-tristis and its effect on combination with Artesunate in Plasmodium berghei K173 induced mice model.
Journal Title: International Journal of Research in Pharmacology & Pharmacotherapeutics (IJRPP) - Year 2018, Vol 7, Issue 1
Abstract
Background Malaria is caused by parasite plasmodium species which is transmitted via the bite of infected mosquitoes, the key approach to treat malaria include prompt and effective treatment with Artemisinin based combination therapy. The search for additional antimalarial from plant extracts and their combination with Artemisinin derivatives must continue to help in effective treatment of the disease. Aim To evaluate the antimalarial efficacy of Nyctanthes arbor-tristis (NAT) and its effect on combination with Artesunate. Materials and Methods The study was done in the Department of Molecular Bioprospection, CSIR-CIMAP, Lucknow, Uttar Pradesh, India. Leaves of Nyctanthes arbor tristis (NAT) were chosen, dried, grounded and extracted with solvent of variable polarity. The bioactivity of NAT leaf extracts was carried out through Plasmodium berghei induced malaria in albino swiss mice animal model. The two independent experiments gave us the result to plan the third experiment on combination therapy. From the first experiment IC50 of Artesunate was derived and from the second experiment the Methanol 80% extract of NAT was found to be indicative of the plant bioactive. The experiment involving the combination therapy was taken up by combining 20mg/kg of Artesunate with 250mg/Kg and 500mg/Kg of Methanol 80% extract of NAT. Results Our study showed that by combining Methanol 80% extract of NAT with sub effective dose of Artesunate results in improvement of mean survival time, parasitaemia and haemoglobin levels. Conclusion Our experiment helped us to conclude that NAT leaves can be used as a lead for combination therapy. However, further detailed experiments related to the safety and pharmacokinetics of combination need to be considered.
Authors and Affiliations
Ashwini Kumar
Keywords
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