Antimycobacterial quinoline alkaloid from the root wood of teclea amaniensis engl.
Journal Title: Scientific Journal of Microbiology - Year 2013, Vol 2, Issue 12
Abstract
Thephytochemical analysis on the root wood of Tecleaamaniensis afforded a quinoline alkaloid veprisine. Its chemical structurewas deduced using Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS) analyses.Veprisine was screened for antimycobacterial activity against two mycobacterialstrains namely; Mycobacterium madagascariense (MM) DSM 44641 and Mycobacterium indicus pranii (MIP) DSM 45239. It exhibited moderate tohigher antimycobacterial activity against test organisms with the MIC values of657.9 µM and 2.63 x 103 µM against MM and MIP respectively.In the same assay isoniazid (INH), a first line anti-TB drug lacked efficacy,even at higher concentration. Consequently, veprisine was further screened todetermine its ability to potentiate the activity of isoniazid against the tworesistant mycobacteria strains. The assay was done by screening the combinationof 1/2 to 1/16 MIC values of veprisine and isoniazid (adopted MIC values of INHagainst M. tuberculosis Mtb H37Rvstrain). Veprisine potentiated the activity of INH against MM and MIP withfractional minimum inhibitory concentration (FMIC) values of 328 µM and 164 µMrespectively. The FMIC values are equivalent to 1/2 and 1/16 MIC values ofveprisine against MM and MIP respectively. When INH and veprisinewere tested alone within this range, they lacked efficacy against the testorganisms. These results show that veprisine is bioactive against Mycobacterium species and it has theability to potentiate the activity of isoniazid against resistant strains.
Authors and Affiliations
P. Erasto*| National Institute for Medical Research, P.O. Box 9653, Dar es Salaam, Tanzania., J. Omolo| National Institute for Medical Research, P.O. Box 9653, Dar es Salaam, Tanzania., C. Hamilton| School of Pharmacy, University of East Anglia, Norwich NR47TJ, United Kingdom., C. d. Koning| Molecular Sciences Institute, School of Chemistry, University of the Witwatersrand, P.O. Wits 2050, Johannesburg, South Africa.
Keywords
Related Articles
- EP ID EP1178
- DOI 10.14196/sjm.v2i12.1003
- Views 510
- Downloads 22
Recurrent appearance of foot and mouth disease virus (FMDV) in Saudi Arabia
There are seven immunologically distinct serotypes of FMDV (O, A, C, Asia 1, SAT 1, SAT 2 and SAT 3), most of them were detected and identified in different regions of Saudi Arabia. FMDV serotype O was the most frequent...
Antibiotic susceptibility profile for salmonella in the Buea health district
Resistance of Salmonella to antibiotics is a serious health problem in developing countries. The aim of this study was to determine the antibiotic susceptibility profile of Salmonella in Buea. Stool samples were coll...
Isolation, molecular detection and BHK-21 adaptation of Newcastle disease virus of field cases in layer farms of Bangladesh
In this study, field samples (n=45) such as trachea, brain and proventriculus were collected from 12 layer farms of different districts of Bangladesh for the detection and isolation of pathogenic Newcastle disease virus...
Distribution and multi-drug resistance pattern of escherichia coli isolated from patients in federal medical center, Owerri
This study was carried out to investigate the distribution and multi-drug resistance (MDR) pattern of Escherichia coli isolated from patients in Federal Medical Centre (FMC), Owerri, Imo State. 255 clinical specimens wer...
Gamma irradiation effects on microbial decontamination of ostrich meat
In this work effects of gamma irradiation and storage time on microbial decontamination of fresh camel meat stored at 4˚C were evaluated. Microbial analysis indicated that irradiation had a significant effect on the redu...