ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF COMBINED EXTRACTS PREPARED USING FICUS RELIGIOSA AND FICUS BENGHALENSIS LEAVES AGAINST CERVICAL CANCER CELL LINE (HELA)
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2018, Vol 11, Issue 12
Abstract
Objectives: Medicinal plants and herbs are used in combination in Ayurveda and folklore medicine as they exhibit good cytotoxic activity. In the present study, the antioxidant, phytochemical, and cell proliferation activity of the combined crude methanolic extract of Ficus religiosa and Ficus benghalensis leaves were investigated.Methods: Antioxidant activity was performed by 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and hydrogen peroxide methods, and the presence of the phytochemicals was screened using the gas chromatography–mass spectrometry. The extract was further evaluated for its cell proliferation activity against cancer cells using the mitochondrial reduction assay. Antioxidant property of the extracts was measured using the DPPH, hydrogen peroxide, and ferric-reducing antioxidant power assay, respectively, using the UV spectrophotometer.Results: The combined extract exhibited strong antioxidant potential in DPPH assay by increase in the percentage of inhibition with the increase in concentration. Similarly, the IC50 value of the methanol extract in peroxidase scavenging activity was 49.85 μg/mL comparatively lower than the ascorbic acid used as standard. The phytochemical analysis of the methanol extract showed the presence of nine phytoconstituents, which exhibit antioxidant and anticancer property. Mitochondrial reduction assay performed to evaluate the cell proliferation activity of the combined leave extract showed that increase in the concentration of the extract decreased the cell proliferation in the HeLa cell line.Conclusion: The results of present study show a possible synergistic activity of leaves against human cervical cancer.
Authors and Affiliations
Suriya Kumaresan, Rema Ramasamy, Philip Robinson Jayachandran
Keywords
Related Articles
- EP ID EP605236
- DOI 10.22159/ajpcr.2018.v11i12.26948
- Views 115
- Downloads 0
A STUDY OF URINE FROM HIV-POSITIVE AND HIV-NEGATIVE INDIVIDUALS AT ANDHRA PRADESH
Objective: The aim of this study is to investigate the variations, if any, in urinary bacterial pathogens in HIV-positive and HIV-negative individuals.Methods: Urine sample, macroscopic and microscopic examination for id...
EFFECT OF DILLENIA INDICA L. AGAINST OXIDATIVE STRESS-INDUCED CARDIOMYOPATHY ON ALLOXAN-INDUCED DIABETES MICE MODEL
 Objective: The aim of the present study is to investigate the antihyperglycemic and antioxidative properties of Dillenia indica fruits.Methods: Aqueous fruit extract and methanolic fruit extract (MFE) were prepared, an...
AN OBSERVATIONAL STUDY OF DRUG USE IN UPPER RESPIRATORY TRACT INFECTION IN PATIENTS ATTENDING ENT OPD IN A TERTIARY CARE HOSPITAL IN KOLKATA
CONTEXT: Majority of upper respiratory tract infections are caused by viruses & thus are self limiting. However, irrational prescription of drugs including antibiotics for URTI is a major problem worldwide. Thus, it...
AN AN OBSERVATIONAL STUDY OF PRESCRIPTION PATTERN OF DRUGS FOR OSTEOARTHRITIS IN A NORTH INDIAN MEDICAL COLLEGE
Objective: The objective of the study is to analyze the prescription pattern of drugs for osteoarthritis (OA) in a north Indian medical college collaborated with a tertiary care hospital. Methods: An observational study...
HIGH SENSITIVE C-REACTIVE PROTEIN AND ITS RELATIONSHIP WITH OTHER CARDIOVASCULAR RISK VARIABLES IN OBESE, OVERWEIGHT AND HEALTHY INDIVIDUALS
Objective: More than 50% of the world’s population is considered overweight and being overweight is associated with several comorbidities such as Type 2 diabetes mellitus, hypertension, cardiovascular diseases, dyslipi...