Antioxidant Property and Phenolic Content of Anthocleista vogelii Root Ethanolic Extract and Fractions
Journal Title: Biotechnology Journal International - Year 2016, Vol 14, Issue 2
Abstract
Aim: To investigate the antioxidant property as well as the phenolic and flavonoid content of Anthocleista vogelii root ethanolic extract (EE) and fractions (aqueous [AF], ethyl acetate [EAF], dichloromethane [DF] and n-hexane [HF] fractions). Study Design: Activity directed antioxidant and phenolic content investigation of A. vogelii ethanolic root extract and fractions using in vitro methods. Place and Duration of the Study: The study was carried out in the Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria between June and August, 2014. Methodology: Evaluation of antioxidant activity was conducted using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS+) free radical scavenging assay. The total Phenolic content and the cupric ions (Cu2+) reducing antioxidant capacity (CUPRAC) of the extracts was determined by spectrophotometric method. Results: The results showed that the ethanolic extract and fractions exerted potent inhibition of DPPH and ABTS+ free radical scavenging activity; EAF (0.23 mg/mL) and DF (1.41 mg/mL) being the most potent respectively. EAF (91.14 mg ascorbic acid equivalent per gram of extract) also exerted a higher CUPRAC when compared with the other extracts. Total Phenol and flavonoid was also present in the ethanolic extract and fractions; DF having the highest content 59.89 mg gallic acid equivalent/g extract and 67.20 mg quercetin equivalents/g extract respectively. Conclusion: The results of this study suggest that A. vogelii ethanolic root extract and fractions have antioxidant potentials and it may be used for the treatment of oxidative stress related diseases including diabetes mellitus.
Authors and Affiliations
Rita M. Sunday, Olapade R. Ilesanmi, Efere M. Obuotor
Keywords
Related Articles
- EP ID EP237979
- DOI 10.9734/BBJ/2016/27016
- Views 118
- Downloads 0
Mitochondrial DNA Variability among Some Saudi Arabian Goat Breeds
Five hundred nucleotides of cytochrome b gene was sequenced for Pakistani, Tihami, Syrian, Masri and Aardi goat breeds inhabiting Saudi Arabia. Based on two computational maximum-parsimony and neighbor-joining methods, t...
Investigation of Genetic Diversity among Medicago species Using RAPD Markers
Aims: Medicago is known as the Queen of forage with potential economic importance to our society. The present study aimed at the use of RAPD-PCR DNA marker to identify the genetic fingerprints affinities of six species o...
Diversity of Bacterial Community in Fermentation of African Oil Bean Seeds (Pentaciethra macrophylla Benth) by comparison of 16S rRNA Gene Fragments
Aims: The microbial diversity, fermentation dynamic and the predominant micro-organisms involved in the fermentation of African oil bean (Pentaciethra macrophylla Benth) seeds to “Ugba” traditional African food in Easter...
Antibacterial Potential of Magnesium Oxide Nanoparticles Synthesized by Aspergillus niger
A total of 280 urinary tract infection samples were collected in this investigation. Out of them 212(75.7%) samples showed a positive response.to bacterial isolates. Morphological, cultural and biochemical testes were co...
Structural Analysis and Docking of Stilbene Synthase Protein from Chinese Grape Vine Vitis pseudoreticulata
Aims: The present work aims to perform the molecular modeling of stilbene synthase protein from Chinese grape vine Vitis pseudoreticulata. Place and Duration of Study: The study has been performed in the Department of Bi...