Antioxidants and Antimicrobial- Activities of Methanol Leaf Extract of Senna occidentalis
Journal Title: Journal of Advances in Medical and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 2
Abstract
Background: Oxidative stress and infectious disease have always been considered as a leading cause of morbidity and mortality in humans. Free radicals damage other molecules by extracting electrons from them in order to attain stability. The side effects associated with commonly used conventional antioxidants drugs have necessitated the search for natural antioxidants from plants extracts. Objective: The antioxidants and antimicrobial activities of methanol leaf extract of S. occidentalis obtained from North Central, Nigeria were evaluated against some pathogenic microorganism. Methods: The antioxidants activities were conducted using DPPH radical scavenging assay. The antibacterial activities were screened against Escherichia coli, Klebsiella pneumoniae and Candida albicans, Staphylococcus aureus, Pseudimonas aeruginosa and Salmonella typhi using agar well diffusion method at various concentrations (80-360 mg/ml). The minimum inhibitory concentration (MIC) was determined using serial dilution method while minimum bacterialcidal concentration (MBC) by plating various dilution of extract. Results: The extract show significant antibacterial activities with MIC in the range of 160 mg/ml- 280 mg/ml and MBC in range of 160 mg/ml – 320 mg/ml. The most sensitive organisms were S. aureus and P. aeruginosa while K. pneumonia show some degree of resistances compared to other organisms tested. However, the organisms were not sensitive to the extract at the dose of 80 and 120 mg/ml. The extracts and the standard antioxidant (Vitamin E) promoted an inhibition of DPPH radical with IC50 value of the extract (263.53±3.24 mg/mL) significantly (p < 0.05) higher than 83.63±2.78 mg/mL obtained for standard drug. Conclusion: Methanol leaf extract of S. occidentalis contains some useful potential antimicrobial and antioxidant compounds that could serve as candidate for the development of drug for therapeutic purposes.
Authors and Affiliations
Ndarubu A. Tsado, Bashir Lawal, Gwadabe N. Kontagora, Balarabe M. Muhammad, Makama A. Yahaya, Jiya A. Gboke, Umar A. Muhammad, Muhammad K. Hassan
Keywords
Related Articles
- EP ID EP328657
- DOI 10.9734/JAMPS/2016/25655
- Views 91
- Downloads 0
Assessment of Risk Involved in the Combination Medicine of Paracetamol and Caffeine
Paracetamol (acetaminophen) with caffeine is a combination medicine indicated for the temporary relief of pain and discomfort associated with a number of conditions such as headache or muscle pain. There is a controversy...
Antiproliferative Activity by Ethanolic Extract of Red Alpinia galanga (L) Willd in Inoculated Breast Carcinoma Cells of C3H Mice
Background: The objective of this study is to investigate the anticancer activity of ethanolic extract of Alpinia galanga Willd (galangal) on breast adenocarcinoma cells transplanted in C3H mice. In some previous studies...
Routine Histopathological Analysis of Non-suspicious Looking Foreskin for Adult Patients: Is It Necessary?
Objective: To evaluate the routine histopathological analysis of foreskin in adult patients and determine whether histological processing is necessary in patients with non-suspicious foreskin. Materials and Methods: Al...
Formulation and In-vitro Evaluation of Ileo-Colonic Targeted Tablet of Flurbiprofen Using Natural and Semi Synthetic Polymers for Ulcerative Colitis
In present research work, we have developed ileo-colonic targeted matrix tablets of flurbiprofen for chronotherapeutic treatment of ulcerative colitis. Direct compression technique was used to formulate matrix tablets of...
Evaluation of the Benefits, Quality of Services and Challenges to the Nigerian National Health Insurance Scheme among Enrollees in a Tertiary Teaching Hospital in Southeast Nigeria
Objective: Access to quality healthcare has been a prerogative of the middle and high socioeconomic class leading to the introduction of health insurance in developing countries to share the burden, reduce cost, and step...