Antiplasmodial Activity of the n-Hexane Extract from Pleurotus ostreatus (Jacq. ex. Fr) P. Kumm.
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2019, Vol 16, Issue 1
Abstract
Objectives: Several mushrooms species have been reported to be nematophagous and antiprotozoan. This study reported the antiplasmodial and cytotoxic properties of the n-hexane extract from the edible mushroom Pleurotus ostreatus and the isolation of a sterol from the extract. Materials and Methods: Antiplasmodial and cytotoxicity assays were done in vitro using the plasmodium lactate dehydrogenase assay and human HeLa cervical cell lines, respectively. The structure of the isolated compound from the n-hexane extract was elucidated using spectroscopic techniques. Results: The n-hexane extract (yield: 0.93% w/w) showed dose dependent antiplasmodial activity with the trend in parasite inhibition of: chloroquine (IC50=0.016 μg/mL) > n-hexane extract (IC50=25.18 μg/mL). It also showed mild cytotoxicity (IC50>100 μg/mL; selectivity index >4) compared to the reference drug emetine (IC50=0.013 μg/mL). The known sterol, ergostan-5,7,22-trien-3-ol, was isolated and characterized from the extract. Conclusion: This study reporting for the first time the antiplasmodial activity of P. ostreatus revealed its nutraceutical potential in the management of malaria.
Authors and Affiliations
Ozadheoghene Eriarie AFIEROHO, Xavier Siwe NOUNDOU, Chiazor P. ONYIA, Osamuyi H. FESTUS, Elizabeth C. CHUKWU, Olutayo M. ADEDOKUN, Michelle ISAACS, Heinrich C. HOPPE, Rui WM. KRAUSE, Kio A. ABO
Keywords
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- EP ID EP684696
- DOI 10.4274/tjps.18894
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