Application of Zinc Oxide Nanoparticles for Fabrication of Biosensor Based on Glucose Oxidase
Journal Title: International Journal of Pharmaceutical and Biological Sciences Fundamentals (www.ijpbsf.com) - Year 2012, Vol 2, Issue 1
Abstract
Nanostructured Zinc oxide (ZnO) is inherently electrocatalytic by nature, thus finding application in the field of enzyme immobilization while also keeping its biological activity undamaged and therefore these nanomaterials are utilized for the construction of biosensors. The electrochemical behavior of zinc oxide is the result of the combined characteristics of electrochemistry as they have high speed, sensitivity, simplicity & also have lower limits of detection. In this particular study we prepared the nanoparticles from metal oxide first and then these zinc oxide nanoparticles are combined with glucose oxidase (GOx). After this step the UV-Spectrum helps in the structural confirmation of GOx. The main reason behind combining zinc oxide nanoparticles with glucose oxidase (GOx) is to enhance the current sensitivity of the GOx enzyme electrode. In 10 mM solution of β-D-glucose we observe the current response of ZnO nanoparticle containing the enzyme electrode increasing from 0.78 to 22 μA cm-2.
Authors and Affiliations
Kamaldeep , Kashyap kumar dubey
Keywords
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