APPLICATION OFF-LINE SPE-HPLC/UV METHODS IN ANALYSIS OF OFLOXACIN IN HUMAN URINE (IN VITRO)
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 1
Abstract
Objective: The objective of this study is to determine the validity of analytical methods in OFX antibiotic study in human urine (in vitro) using an SPE-HPLC/UV. In this study, SPE was applied in preparing the analysis of ofloxacin using HPLC embedded UV detector.Methods: C-18 (octadecylsilane) cartridge (100 mg, particle size 10 µm) of SPE was employed in preparing a sample to determine further of analytes using HPLC with phosphate buffer 0.025 M (pH 2.5) and acetonitrile (85.5:14.5) as mobile phase and a flow rate of 1.2 m l/min. UV detector was adjusted at 295 nm with the internal standard ciprofloxacin.Results: The calibration curves for the ofloxacin were linear over concentrations ranging from 1.15 to 36.0 µg/ml with a correlation coefficient (r) from 0.9998 to 0.9999. The coefficients of variation obtained from ofloxacin were less than 10 %. Ofloxacin on the area ratio of peak height and a segment of the chromatogram, LOD and LOQ of ofloxacin were 0.12 and 0.4 μg/ml, respectively. The recovery of ofloxacin from spiked human urine was 96.0 %.Conclusion: The validation methods that including parameters: selectivity, repeatability, linearity, detection limit, quantification limit, precision, accuracy, and suitability of the system. The methods used have validity according to the requirements that might be used to analyze ofloxacin in human urine.Â
Authors and Affiliations
Muchtaridi Muchtaridi, Ester Yuliani, Iyan Sopyan
Keywords
Related Articles
- EP ID EP577949
- DOI -
- Views 78
- Downloads 0
INSILICO ANALYSIS OF DIETARY AGENTS AS ANTICANCER INHIBITORS OF INSULIN LIKE GROWTH FACTOR 1 RECEPTOR (IGF1R)
Objective: Insulin-like growth factor 1 receptor (IGF-1R) is over-expressed in a number of cancer cell lines and has been implicated to play a role in oncogenesis and suppression of apoptosis. Thus, the inhibition of IGF...
SCAR MARKER DEVELOPMENT FOR THE CORRECT IDENTIFICATION OF IRIS ENSATA
Objective: The objective of this research was to develop the RAPD based SCAR marker for the correct identification of the Iris ensata Thunb. (I. ensata) plant from its adulterants.Methods: Five samples of I. ensata. from...
BIO-HERBICIDAL EFFICIENCY OF WITHANIA SOMNIFERA AGAINST IMPORTANT HIMALAYAN WEEDS
Objective: The aim of the study was to evaluate the bioherbicidal activity of Withania somnifera against germination and growth of noxious weeds of Himalaya i.e. Ageratum coenyzoides, Chenopodium album and Achyranthus as...
THE EFFECTS OF HOMOCYSTEINE ON PLASMA BIOCHEMICAL PARAMETERS AND AORTIC MATRIX METALLOPROTEINASES ACTIVITIES
Objective; Increased levels of physiological amino acid homocysteine (Hcy) in plasma is associated with the development of cardiovascular, neuronal, and liver diseases.[1] Damage of the vascular wall of aorta develops by...
CURCUMIN-LOADED MULTI-VALENT LIGANDS CONJUGATED-NANOPARTICLES FOR ANTI-INFLAMMATORY ACTIVITY
Objective: The present study aims to develop curcumin-loaded muti-valent ligands conjugated-nanoparticlesfor targeted cell and study the biological activities including anti-inflammatory activity and kinetic cellular upt...