Arachis hypogaea L. Direct Organogenesis and SDS - PAGE Analysis in Groundnut
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 6
Abstract
The present research work was carried out to observe the effect of various concentrations of Benzyl amino purine (BAP) in terms of multiple shoot induction with special emphasis on qualitative and quantitative changes of proteins during multiple shoot formation. From whole embryonated cotyledons of groundnut. The shoot induction medium containing different concentrations of Benzyle amino purine ranged from 5.0 to 25.0mg/l along with lower concentration of Indole acetic acid (IAA).The multiple shoots were observed in all the BAP concentrations at a varying levels. Among the various levels of BAP, 25.0mg/l of BAP plus 0.50mg/l of IAA was found to be the most effective in terms of multiple shoot induction. The growth parameters like plantlet height, fresh and dry weight also highly influenced by the concentration of the BAP. The root induction was achieved in micropropagated shoots by using Indole butyric acid (IBA) at a concentrations of 1.0 to 5.0mg/l. The mean number of roots, root length was highly influenced by IBA. In all the concentrations of IBA the rooting was associated with moderate and heavily associated basal callusing. The quantitative estimation of protein content in regenerated shoots also increased with higher concentrations of BAP. The SDS-PAGE analysis showed that there was a nine visible bands with 82.2, 76.4, 57.5, 27.6, 23.7, 21.4, 18.2, 16.5 and 12.8 kDa were observed in multiple shoots derived from all the concentrations of BAP. Whereas in whole embryonated cotyledonary explants there was a entirely different protein banding pattern with 82.2, 76.4, 57.5, 27.6, 23.7, 21.4, 18.2, 16.5 and 12.8 kDa were recorded.
Authors and Affiliations
S. Palanivel, V. Muniappan, C. Deepa, A. Abuthahir
Keywords
Related Articles
- EP ID EP237680
- DOI 10.9734/BBJ/2014/9237
- Views 119
- Downloads 0
Effect of Gas Flaring on the Phytochemical and Nutritional Composition of Treculia africana and Vigna subterranean
Aims: The effect of petroleum exploration and exploitation activities on the phytochemical and nutritional composition of African breadfruit (Treculia africana) and Bambara groundnuts (Vigna subterranean) harvested from...
Database Analysis of Acidic Proteins from Halophilic Species and Their Corresponding Basic Proteins from Non-halophilic Species
Aims: To reveal which amino acid residues determine whether a protein is acidic or basic between orthologous pairs, acidic proteins from halophilic species and corresponding basic proteins from non-halophilic species wer...
Application of Box-Behnken Design for the Optimized Production of Lactic Acid by Newly Isolated Lactobacillus plantarum JX183220 Using Cassava (Manihot esculenta Crantz) Flour
Aim: The present study aimed at optimization of Lactic acid production using new isolate, Lactobacillus plantarum JX183220 with cassava flour (Manihot esculenta Crantz) in semi-solid fermentation by Response Surface Meth...
Stability Evaluation and Degradation Kinetics of Ascorbic Acid in Baobab Fruit Pulp Formulated with the Seed Oil
Aims: Baobab (Adansonia digitata) fruit pulp and the seed oil contain appreciable amount of vitamins and nutrients which help fight off diseases and afford commendable source of nourishment. It is essential to screen for...
Somatic Embryogenesis and Plantlet Regeneration from Protoplast Culture of Stevia rebaudiana
In order to develop a high-efficiency and reproducible regeneration protocol for Stevia protoplasts, various factors such as type and concentration of enzymes, osmoticum, incubation time, plant material type and age were...