Arsenic induces oxidative stress and apoptosis in human lung cancer cells
Journal Title: International Journal of Life Science - Year 2017, Vol 5, Issue 4
Abstract
Arsenic is a toxic heavy metal and a well-known environmental contaminant. Often water, food, soil are found to be contaminated with arsenic and occupational exposure leads to harmful health issues. Hence, in the present study human lung epithelial A549 cells were used as in vitro model system. Treatment of A549 cells with arsenic for 48 h showed reduced viability with increased leakage of lactate dehydrogenase with loss of membrane damage. Arsenic significantly induces reactive oxygen species with depletion of free radical neutralizing antioxidant glutathione peroxidase and catalase enzymes. IC50 value of 2 µM arsenic concentration was used for mRNA expression studies. Further, pro-apoptotic Bax gene was induced and anti-apoptotic Bcl-2 was decreased. Arsenic stimulates dysfunction of cell cycle progression by downregulation of Cyclin-E1and Cyclin-D1 mRNA expressions. Thus confirms the toxic nature of arsenic and clues to oxidative redox potential alteration with cell cycle inhibition and apoptosis in lung epithelial cells.
Authors and Affiliations
Kiran Kumar KM, Naveen Kumar M, Nagesh Rashmi, Rajeshwari H Patil, Babu RL, S Chidananda Sharma, K Manjunath
Keywords
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